In Vivo Effects of Ozone Exposure on Protein Adduct Formation by 1-Nitronaphthalene in Rat Lung
| Autor: | Dexter Morin, Teresa C. Wegesser, Alan R. Buckpitt, Bridget C. Boland, Åsa M. Wheelock, Charles G. Plopper, Margaret A. Isbell |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Proteomics Pulmonary and Respiratory Medicine Antioxidant medicine.medical_treatment Clinical Biochemistry Naphthalenes medicine.disease_cause Article Adduct Rats Sprague-Dawley Ozone In vivo medicine Animals Lung Molecular Biology Air Pollutants biology Chemistry Biliverdin reductase Proteins Cell Biology Rats Oxidative Stress Biochemistry Toxicity biology.protein Respiratory epithelium Calreticulin Oxidative stress |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology. 33:130-137 |
| ISSN: | 1535-4989 1044-1549 |
| DOI: | 10.1165/rcmb.2005-0047oc |
| Popis: | The incidence of serious photochemical smog events is steadily growing in urban environments around the world. The electrophilic metabolites of 1-nitronaphthalene (1-NN), a common air pollutant in urban areas, have been shown to bind covalently to proteins. 1-NN specifically targets the airway epithelium, and the toxicity is synergized by prior long-term ozone exposure in rat. In this study we investigated the formation of 1-NN protein adducts in the rat airway epithelium in vivo and examined how prior long-term ozone exposure affects adduct formation. Eight adducted proteins, several involved in cellular antioxidant defense, were identified. The extent of adduction of each protein was calculated, and two proteins, peroxiredoxin 6 and biliverdin reductase, were adducted at high specific activities (0.36–0.70 and 1.0 nmol adduct/nmol protein). Furthermore, the N-terminal region of calreticulin, known as vasostatin, was adducted only in ozone-exposed animals. Although vasostatin was adducted at relatively low specific activity (0.01 nmol adduct/nmol protein), the adduction only in ozone-exposed animals makes it a candidate protein for elucidating the synergistic toxicity between ozone and 1-NN. These studies identified in vivo protein targets for reactive 1-NN metabolites that are potentially associated with the mechanism of 1-NN toxicity and the synergistic effects of ozone. |
| Databáze: | OpenAIRE |
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