Studies of controlled reperfusion after ischemia
| Autor: | Michael P. Sherman, Christophe Acar, Marshall T. Partington, Pierre Julia, Gerald D. Buckberg |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pulmonary and Respiratory Medicine
Radical Ischemia Anterior Descending Coronary Artery Superoxide dismutase medicine Carnivora chemistry.chemical_classification biology business.industry Glutathione peroxidase Fissipedia medicine.disease biology.organism_classification Oxygen tension medicine.anatomical_structure chemistry Catalase Coronary occlusion Anesthesia Room air distribution biology.protein Surgery Leukocyte depletion Cardiology and Cardiovascular Medicine Ligation business Artery |
| Zdroj: | The Journal of Thoracic and Cardiovascular Surgery. 101:350-359 |
| ISSN: | 0022-5223 |
| Popis: | Postischemic damage is caused partially by oxygen free radical-mediated injury. This study will show that (1) crystalloid cardioplegia with room air oxygen is deleterious because it is devoid of free radical scavengers and (2) blood cardioplegia limits damage because it contains endogenous free radical scavengers in red blood cells. Methods: Thirty-two dogs underwent 2 hours of ligation of the left anterior descending coronary artery followed by 20 minutes of regional blood cardioplegic reperfusion on bypass. Ten dogs received only the blood cardioplegic solution (containing its endogenous free radical scavengers); five received initial blood cardioplegia (5 minutes) with endogenous free radical scavengers (catalase and glutathione peroxidase) blocked by aminotriazole and N-ethylmaleimide, respectively; 12 received initial crystalloid cardioplegic solution oxygenated by room air (oxygen tension = 150 mm Hg); seven without and five with exogenous free radical scavengers (superoxide dismutase, catalase, coenzyme Q10); five received initial deoxygenated crystalloid cardioplegic solution (oxygen tension = 6 mm Hg); and five received deoxygenated crystalloid cardioplegic solution. Results: Blood cardioplegia with endogenous free radical scavengers produced the best recovery of systolic shortening (69% systolic shortening) and resulted in the least histochemical damage (11% triphenyltetrazolium chloride nonstaining). The worst recovery and most damage occurred if blood cardioplegia was preceded by oxygenated crystalloid cardioplegia (3% systolic shortening, 48% triphenyltetrazolium chloride nonstaining; p |
| Databáze: | OpenAIRE |
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