Glycogen Synthase Kinase-3β (GSK-3β) and Nuclear Factor Kappa-B (NFKB) in Childhood Acute Lymphoblastic Leukemia
| Autor: | María del Socorro Camarillo Romero, Isidoro Tejocote Romero, Cristian Fabian Layton Tovar, Yanko Valentin Fabila Sanchez, Hugo Mendieta Zerón |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Prognostic variable Adolescent Medicine (miscellaneous) Peripheral blood mononuclear cell Gastroenterology General Biochemistry Genetics and Molecular Biology Immunophenotyping 03 medical and health sciences 0302 clinical medicine GSK-3 Internal medicine Acute lymphocytic leukemia Biomarkers Tumor Internal Medicine medicine Humans Pharmacology (medical) Child Childhood Acute Lymphoblastic Leukemia Genetics (clinical) Glycogen Synthase Kinase 3 beta Gene Expression Regulation Leukemic business.industry NF-kappa B Precursor Cell Lymphoblastic Leukemia-Lymphoma medicine.disease Immunohistochemistry 030104 developmental biology medicine.anatomical_structure Endocrinology Child Preschool 030220 oncology & carcinogenesis Reviews and References (medical) Female Bone marrow business |
| Zdroj: | Advances in Clinical and Experimental Medicine. 25:1139-1147 |
| ISSN: | 1899-5276 |
| Popis: | BACKGROUND Acute lymphocytic leukemia (ALL) is the most common hematologic malignancy in early childhood. In children with acute lymphoblastic leukemia (ALL), the activity of glycogen synthase kinase (GSK-3β) has been associated with changes in the transcriptional activity and expression of nuclear factor kappa beta (NFKB) in the mononuclear cells of bone marrow. OBJECTIVES The aim of the study was to determine the possible role of glycogen synthase kinase 3beta (GSK-3β) and nuclear factor kappa beta (NFKB) as prognostic variables in pediatric patients with ALL. MATERIAL AND METHODS This was a descriptive, transversal, and observational study. Bone marrow and blood samples were obtained from 30 children with newly-diagnosed ALL, who were seen at the Hematology-Oncology Service, Hospital para el Nino (HPN), Toluca, Mexico, from 2014‒2015. Anthropometric variables, clinical lab results, immunophenotype and cytogenetic abnormalities were registered. GSK-3β was evaluated through immunohistochemistry, and NFKB messenger RNA (mRNA) with real-time polymerase chain reaction (qPCR). The cases of ALL were classified into two groups of risk: high and habitual. RESULTS Thirty patients were included in this study, with a mean age of 7.1 years (range 2‒13 years). Twenty-one were male and 9 female. Employing the morphological classification, 26 patients had type L1 ALL and the remaining 4 patients had type L2 ALL. Abnormal genes were found in 7 (23.33%) patients, ETV-RUNX1 in 3, followed by TCF3-PBX1 (two), STL1-TAL1 (one), and BCR-ABL1 (one). NFKB relative expression levels, in comparison to the GSK-3β immunohistochemistry results of the bone marrow samples, showed significant differences between positive and negative cases (p = 0.001) and between weak-positive and negative cases (p = 0.002). CONCLUSIONS These results suggest that GSK-3β may be a prognostic biomarker in childhood ALL. |
| Databáze: | OpenAIRE |
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