Fate of3H- and14C-labelled A-4166 in pancreatic islets
| Autor: | W. J. Malaisse, F. Malaisse-Lagae |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
endocrine system
medicine.medical_specialty Phenylalanine Endocrinology Diabetes and Metabolism media_common.quotation_subject Nateglinide Tritium Islets of Langerhans Endocrinology Cyclohexanes Internal medicine Internal Medicine medicine Animals Hypoglycemic Agents Carbon Radioisotopes Rats Wistar Internalization Receptor Incubation Cells Cultured media_common geography geography.geographical_feature_category Chemistry Pancreatic islets General Medicine Metabolism Islet Rats Meglitinide medicine.anatomical_structure Mechanism of action Autoradiography Female medicine.symptom |
| Zdroj: | Acta Diabetologica. 33:298-300 |
| ISSN: | 1432-5233 0940-5429 |
| Popis: | The fate of 3H- and 14C-labelled A-4166 was examined in rat pancreatic islets. The net uptake of the meglitinide analogue by islets incubated for 60 min in the presence of 0.1 mM A-4166 and then submitted to repeated washes was close to 0.1 pmol/islet. It was significantly increased when the concentration of D-glucose in the incubation medium was raised from 2.8 to 16.7 mM. No sizeable internalization of tritiated A-4166 into insulin-producing cells could be detected by autoradiography. These findings suggest that the interaction of A-4166 with the beta-cell may be restricted to its insertion on the plasma membrane and binding to sulphonylurea receptors. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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