Laser desorption/ionization mass spectrometry of lipids using etched silver substrates
| Autor: | Klaus Dreisewerd, Ann-Christin Niehoff, Andreas Schnapp, Annika Koch |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Silver Nanoparticle Metal Nanoparticles Mass spectrometry Photochemistry 01 natural sciences General Biochemistry Genetics and Molecular Biology Adduct 03 medical and health sciences Ionization Desorption Animals Molecular Biology Wax Chromatography Chemistry Lasers fungi 010401 analytical chemistry Substrate (chemistry) Bees Lipids 0104 chemical sciences 030104 developmental biology Drosophila melanogaster visual_art Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization visual_art.visual_art_medium Mass spectrum |
| Zdroj: | Methods (San Diego, Calif.). 104 |
| ISSN: | 1095-9130 |
| Popis: | Silver-assisted laser desorption/ionization mass spectrometry can be used for the analysis of small molecules. For example, adduct formation with silver cations enables the molecular analysis of long-chain hydrocarbons, which are difficult to ionize via conventional matrix-assisted laser desorption ionization (MALDI). Here we used highly porous silver foils, produced by etching with nitric acid, as sample substrates for LDI mass spectrometry. As model system for the analysis of complex lipid mixtures, cuticular extracts of fruit flies (Drosophila melanogaster) and worker bees (Apis mellifera) were investigated. The mass spectra obtained by spotting extract onto the etched silver substrates demonstrate the sensitive detection of numerous lipid classes such as long-chain saturated and unsaturated hydrocarbons, fatty acyl alcohols, wax esters, and triacylglycerols. MS imaging of cuticular surfaces with a lateral resolution of a few tens of micrometers became possible after blotting, i.e., after transferring lipids by physical contact with the substrate. The examples of pheromone-producing male hindwings of the squinting bush brown butterfly (Bicyclus anynana) and a fingermark are shown. Because the substrates are also easy to produce, they provide a viable alternative to colloidal silver nanoparticles and other so far described silver substrates. |
| Databáze: | OpenAIRE |
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