Human Herpesvirus 8 Infection in Patients With Cutaneous Lymphoproliferative Diseases
| Autor: | A. Mastroianni, Carmela Ferraro, Luca Muscardin, Ilaria Lesnoni La Parola, Valentina Bordignon, Franco Ameglio, A. Mussi, Concetta Castilletti, Giovanna D'Agosto, Paola Cordiali-Fei, Ada Amantea, Joachim W. Fluhr, Elisabetta Trento |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Population Lymphoproliferative disorders Genome Viral Dermatology Antibodies Viral Skin Diseases Lesion Open Reading Frames Mycosis Fungoides Seroepidemiologic Studies Prevalence Humans Psoriasis Medicine Gammaherpesvirinae education Sarcoma Kaposi Aged Gene Rearrangement Mycosis fungoides education.field_of_study biology Parapsoriasis business.industry Infant Newborn Infant Receptors Antigen T-Cell gamma-delta Herpesviridae Infections General Medicine Gene rearrangement Middle Aged medicine.disease biology.organism_classification Immunohistochemistry Lymphoproliferative Disorders Case-Control Studies Child Preschool Herpesvirus 8 Human Immunology medicine.symptom business Nested polymerase chain reaction |
| Zdroj: | Archives of Dermatology. 141 |
| ISSN: | 0003-987X |
| DOI: | 10.1001/archderm.141.10.1235 |
| Popis: | Objective To investigate the prevalence of human herpesvirus 8 (HHV-8; Kaposi sarcoma–associated herpesvirus) infection in patients with lymphoproliferative skin diseases such as large-plaque parapsoriasis (LPP) and mycosis fungoides compared with inflammatory cutaneous conditions or healthy control subjects. Design A survey study was undertaken in 123 subjects with various clinical conditions. Setting All patients had been seen in the Dermatology Department of the San Gallicano Dermatology Institute, Rome, Italy, in the last 2 years. Patients Forty-five patients with inflammatory or autoimmune cutaneous diseases, 50 healthy control subjects, 10 patients with LPP, 12 patients with mycosis fungoides, and 6 patients with classic Kaposi sarcoma were included in the study. Main Outcome Measures The prevalence of HHV-8 infection was investigated with serologic studies using the gold standard assay based on body cavity–based B-cell lymphoma-1 cells latently infected with HHV-8. The presence of HHV-8 conserved sequence, corresponding to open reading frame 26, was also assessed in the peripheral blood and lesion tissue samples from patients with lymphoproliferative cutaneous diseases with nested polymerase chain reaction. The presence and distribution of cell types infected with HHV-8 in the lesion tissues was determined with immunohistochemical staining with the monoclonal antibody directed against the latent nuclear antigen-1 of HHV-8 encoded by open reading frame 73. Results In healthy control subjects and patients with inflammatory skin diseases, 13.9% were found to have antibody against HHV-8, consistent with the seroprevalence population in Italy. A highly significant association of HHV-8 infection and LPP was found (100%) compared with mycosis fungoides (25%). The peripheral blood mononuclear cells in 8 of 10 patients with LPP were found to harbor viral sequences at nested polymerase chain reaction, whereas none of them had a detectable serum viral load. All LPP lesion tissue samples were positive for HHV-8–encoded open reading frame 26, and the presence of HHV-8–infected cells was confirmed by immunohistochemistry profiles performed on paraffin-embedded tissues from 4 of 10 patients. The positive cell types included endothelial cells and the infiltrating dermal lymphocytes, characteristic hallmarks of LPP. Analysis of T-cell receptor γ chain rearrangements in lesion tissue from our patients confirmed the lack of a significant association between T-cell clonality and LPP. Conclusion These data suggest that HHV-8 may play a role in the onset of LPP, a disease whose cause and evolution are still undefined and which has often been considered the early stage of mycosis fungoides. |
| Databáze: | OpenAIRE |
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