Identification of DELE, a novel DAP3-binding protein which is crucial for death receptor-mediated apoptosis induction
| Autor: | Tanenobu Harada, Atsushi Iwai, Tadaaki Miyazaki |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Ribosomal Proteins
Cancer Research Clinical Biochemistry Pharmaceutical Science Gene Expression Caspase 3 Apoptosis Caspase 8 Cell Line Mitochondrial Proteins TNF-Related Apoptosis-Inducing Ligand Humans RNA Small Interfering Caspase Pharmacology DAP3 Gene knockdown biology Tumor Necrosis Factor-alpha Binding protein Biochemistry (medical) Proteins RNA-Binding Proteins Cell Biology Receptors Death Domain Molecular biology Caspase 9 Cell biology Mitochondria Gene Knockdown Techniques biology.protein Signal transduction Apoptosis Regulatory Proteins HeLa Cells Signal Transduction |
| Zdroj: | Apoptosis : an international journal on programmed cell death. 15(10) |
| ISSN: | 1573-675X |
| Popis: | Death associated protein 3 (DAP3) is known to be a highly conserved protein, and is responsible for regulating apoptosis induced by various stimuli. To understand the molecular mechanism of how DAP3 induces apoptosis, we performed yeast two-hybrid screening, and identified a novel DAP3-binding protein termed death ligand signal enhancer (DELE). In this report, we show that DELE actually binds to DAP3 in mammalian cells. We found that the cells stably expressing DELE are susceptible to apoptosis induction by the stimulation of TNF-α and TRAIL. In addition, knockdown of DELE expression rescued the HeLa cells from apoptosis induction by these stimuli. Moreover, activation of caspase-3, caspase-8 and caspase-9 induced by stimulation of TNF-α, anti-Fas or TRAIL was significantly inhibited by the knockdown of DELE expression. These results demonstrated the biological significance of DELE for apoptosis signal mediated by death receptors. |
| Databáze: | OpenAIRE |
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