Evaluation of CERS2 Gene as a Potential Biomarker for Bladder Cancer

Autor: Rosna Yunus, Aminuddin Baharudin, Umar Ahmad, Soon Choy Chan, Teng Aik Ong, Azad Hassan Abdul Razack, Khatijah Yusoff, Abhi Veerakumarasivam, Ahmed Faris Aldoghachi
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
Article Subject
Clinical Biochemistry
Newcastle disease virus
03 medical and health sciences
Inhibitory Concentration 50
0302 clinical medicine
Downregulation and upregulation
Cell Movement
Cell Line
Tumor
Sphingosine N-Acyltransferase
Genetics
Biomarkers
Tumor
Medicine
Humans
RNA
Small Interfering
Molecular Biology
Regulation of gene expression
Oncolytic Virotherapy
lcsh:R5-920
Bladder cancer
business.industry
Tumor Suppressor Proteins
Biochemistry (medical)
Ceramide synthase 2
Cancer
Membrane Proteins
General Medicine
medicine.disease
Oncolytic virus
Gene Expression Regulation
Neoplastic
030104 developmental biology
Phenotype
Urinary Bladder Neoplasms
Cell culture
030220 oncology & carcinogenesis
Cancer research
Immunohistochemistry
Neoplasm Recurrence
Local
lcsh:Medicine (General)
business
Research Article
Zdroj: Disease Markers
Disease Markers, Vol 2019 (2019)
ISSN: 1875-8630
0278-0240
Popis: The ceramide synthase 2 (CERS2) gene has been linked to tumour recurrence and invasion in many different types of cancers including bladder cancer. In this study, the expression levels of CERS2 in bladder cancer cell lines were analysed using qRT-PCR and the protein expression in clinical bladder cancer histopathological specimens were examined via immunohistochemistry. The potential utility of CERS2 as a predictive biomarker of response to oncolytic virotherapy was assessed by correlating the CERS2 mRNA expression to IC50 values of cells treated with the Newcastle disease virus (NDV), AF2240 strain. This study demonstrates that CERS2 is differentially expressed in different types of bladder cancer cell lines and that the siRNA-mediated downregulation of the expression of CERS2 reduces the migratory potential of UMUC1 bladder cancer cells. However, there were no significant correlations between the expression levels of the CERS2 protein with bladder cancer grade/stage or between the IC50 values of cells treated with NDV and CERS2 expression. Although the utility of CERS2 expression may be limited, its potential as an antimigration cancer therapeutic should be further examined.
Databáze: OpenAIRE
Externí odkaz: