Week-long imaging of cell divisions in the Arabidopsis root meristem

Autor: Kenneth D. Birnbaum, Ramin Rahni
Rok vydání: 2019
Předmět:
Zdroj: Plant Methods, Vol 15, Iss 1, Pp 1-14 (2019)
Plant Methods
ISSN: 1746-4811
Popis: Background Characterizing the behaviors of dynamic systems requires capturing them with high temporal and spatial resolution. Owing to its transparency and genetic tractability, the Arabidopsis thaliana root lends itself well to live imaging when combined with cell and tissue-specific fluorescent reporters. We developed a novel 4D imaging method that utilizes simple confocal microscopy and readily available components to track cell divisions in the root stem cell niche and surrounding region for up to 1 week. Results Using this method, we performed a direct measurement of cell division intervals within and around the root stem cell niche. The results reveal a short, steep gradient of cell division rates in proximal stem cells, with progressively more rapid cell division rates from quiescent center (QC), to cells in direct contact with the QC (initials), to their immediate daughters, after which division rates appear to become more homogeneous. Conclusions These results provide a baseline to study how perturbations in signaling could affect cell division patterns in the root meristem. This new setup further allows us to finely analyze meristematic cell division rates that lead to patterning. Electronic supplementary material The online version of this article (10.1186/s13007-019-0417-9) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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