Expression of platelet-activating factor receptor transcript-2 is induced by shear stress in HUVEC
| Autor: | Morito Monden, Kazuhiro Okahara, Tomio Kawasaki, Jun-ichi Kambayashi, Bing Sun |
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| Rok vydání: | 1998 |
| Předmět: |
Umbilical Veins
Physiology Receptor expression Inflammation Receptors Cell Surface Platelet Membrane Glycoproteins Platelet membrane glycoprotein Biochemistry Polymerase Chain Reaction Receptors G-Protein-Coupled Stress Physiological Physical Stimulation medicine Humans RNA Messenger Receptor Cells Cultured Pharmacology Regulation of gene expression Chemistry Alternative splicing Cell Biology Sequence Analysis DNA Flow Cytometry Cell biology Endothelial stem cell Alternative Splicing Gene Expression Regulation Immunology lipids (amino acids peptides and proteins) Endothelium Vascular medicine.symptom Platelet-activating factor receptor |
| Zdroj: | Prostaglandinsother lipid mediators. 55(5-6) |
| ISSN: | 1098-8823 |
| Popis: | Platelet-activating factor (PAF) is an important lipid involved in inflammation reaction and circulation regulation. The receptor for human PAF is synthesized from two spliced transcripts of the same gene. Our observation in the present study shows that HUVEC express transcript-1 only in the static condition. Shear stress induces the expression of transcript-2 in these cells but not transcript-1, resulting in increased PAF receptor expression as measured by FACS analysis. These results suggest that shear stress may increase the susceptibility of endothelial cells to PAF by inducing transcript-2 expression. |
| Databáze: | OpenAIRE |
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