Epitope mapping of the Dermatophagoides pteronyssinus house dust mite major allergen Der p II using overlapping synthetic peptides
| Autor: | P. C. Driedijk, R. C. Aalberse, Günther Jung, M. van den Berg, W van 't Hof, A G Beck-Sickinger |
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| Rok vydání: | 1991 |
| Předmět: |
Antigenicity
Immunology Molecular Sequence Data Peptide medicine.disease_cause Epitope Antigen-Antibody Reactions Epitopes Allergen Radioallergosorbent Test medicine Animals Humans Amino Acid Sequence Antigens Dermatophagoides Molecular Biology Peptide sequence House dust mite chemistry.chemical_classification Mites biology Chemistry Chromosome Mapping Allergens Immunoglobulin E biology.organism_classification Epitope mapping Biochemistry biology.protein Antibody |
| Zdroj: | Molecular immunology. 28(11) |
| ISSN: | 0161-5890 |
| Popis: | Fourteen synthetic peptides of 15 amino acid residues length, overlapping by five residues and spanning the entire sequence of the major allergen Der p II from the house dust mite Dermatophagoides pteronyssinus were synthesized. These peptides were coupled to CNBr-activated Sepharose-4B and used as solid-phase antigens in epitope mapping studies using human IgE antisera. These antibodies bound predominantly to the peptide comprising residues 65-78, the binding of which was inhibited by native Der p II. In addition these antisera bound, to a lesser extent, to the peptide that comprised residues 1-15, which binding was not inhibited by native Der p II. Thus, we found one sequential epitope for a number of IgE sera. |
| Databáze: | OpenAIRE |
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