Generation of goats by nuclear transfer: a retrospective analysis of a commercial operation (1998-2010)
| Autor: | D Bonzo, Li How Chen, N. Buzzell, Daniel Pollock, William G. Gavin, N Hawkins, K. Miner, Harry M. Meade, Catherine A. Porter, S Blash |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Nuclear Transfer Techniques animal structures Offspring viruses Cloning Organism Cell Biology 01 natural sciences Cryopreservation Andrology Animals Genetically Modified 03 medical and health sciences Fetus Genetics medicine Animals Animal Husbandry Retrospective Studies Cloning Goats fungi Commerce Embryo Transfection Embryo Transfer Embryo Mammalian 030104 developmental biology medicine.anatomical_structure Blastocyst Cell culture embryonic structures Somatic cell nuclear transfer Animal Science and Zoology Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | Transgenic research. 29(4) |
| ISSN: | 1573-9368 |
| Popis: | At LFB USA, Inc., the ultimate use for transgenic cloned goats is for the production of recombinant human protein therapeutics in their milk. This retrospective analysis of the Somatic Cell Nuclear Transfer (SCNT) program, spanning from 1998 to 2010, examined parameters potentially affecting the outcomes and efficiencies in this commercial operation. Over 37,000 + ova were utilized in the SCNT protocol producing a total of 203 cloned goats. Fifty one (51) clones were produced from non-transfected (transgenic and non-transgenic animal donor) cell lines and 152 clones were produced from transfected cell lines. Comparisons and summaries of (a) transfected versus non-transfected cell lines, (b) relationship of SCNT parameters to offspring produced, (c) skin versus fetal cells, (d) fresh versus cryopreserved cells, (e) parameters from all cell lines used versus those producing SCNT offspring, (f) variation among cell sources, (g) methods of SCNT parturition management and effects on live offspring, and lastly (h) SCNT variation by program are reported. Findings indicate that (a) non-transfected cell lines were more efficient versus transfected cell lines in generating viable cloned offspring on a per reconstructed embryo transferred basis, (b) transfected fetal fibroblasts had improved efficiency versus transfected skin fibroblasts, (c) the percentage of non-transfected cell lines that produced offspring was statistically higher than transfected cell lines, (d) and induction of parturition improved the percentage of viable offspring. In summary, this retrospective analysis on the SCNT process has identified certain parameters for improved efficiency in producing viable cloned goats in a commercial setting. |
| Databáze: | OpenAIRE |
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