Histone acetylation during the in vitro maturation of bovine oocytes with different levels of competence
| Autor: | S. A. D. Rodrigues, T. P. Pontelo, Maurício Machaim Franco, Margot Alves Nunes Dode, Márcio Gilberto Zangeronimo, Alexandre Cavallieri Gomes, Ligiane O Leme, T. S. Kawamoto |
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| Rok vydání: | 2019 |
| Předmět: |
Reproductive technology
Histone H4 Andrology Histones 03 medical and health sciences 0302 clinical medicine Endocrinology Oogenesis Genetics Animals RNA Messenger Molecular Biology 030304 developmental biology Histone Acetyltransferases 0303 health sciences 030219 obstetrics & reproductive medicine Cumulus Cells biology Histone deacetylase 2 Lysine Acetylation Histone acetyltransferase In vitro maturation In Vitro Oocyte Maturation Techniques Histone Reproductive Medicine biology.protein Oocytes Animal Science and Zoology Cattle Female HAT1 Developmental Biology Biotechnology |
| Zdroj: | Reproduction, fertility, and development. 32(7) |
| ISSN: | 1031-3613 |
| Popis: | We aimed to analyse the histone acetylation status and expression profile of genes involved in histone acetylation (histone acetyltransferase 1 (HAT1), lysine acetyltransferase 2A (KAT2A), histone deacetylase 1(HDAC1), HDAC2 and HDAC3) in bovine oocytes of different competences during invitro maturation (IVM). Cumulus–oocyte complexes were recovered from two groups of follicles: minor follicles (1.0–3.0mm in diameter), classified as low competence (LC) and large follicles (6.0–8.0mm in diameter) classified as high competence (HC). Oocytes were submitted to IVM for 0, 8 and 24h and stored for analysis. Acetylation status of histone H4 on lysine K5, K6, K12 and K16 was assessed by immunohistochemistry. For gene expression, mRNA levels were determined by real-time quantitative polymerase chain reaction. All oocytes, regardless of their competence, showed a gradual decrease (P |
| Databáze: | OpenAIRE |
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