Preparation of specimens of human serum gamma-globulin modified by copper and zinc and its immunochemical characteristics
| Autor: | E. M. Mongush, E. A. Denisova, E. E. Babaeva, S. B. Cheknev, U. A. Vorob’eva |
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| Rok vydání: | 2007 |
| Předmět: |
Radial immunodiffusion
Chromatography Protein Conformation chemistry.chemical_element Proteins Ultrafiltration Gamma globulin Enzyme-Linked Immunosorbent Assay General Medicine Plasma protein binding Zinc Copper Immunohistochemistry General Biochemistry Genetics and Molecular Biology Ultrafiltration (renal) Protein structure chemistry Cations Immunochemistry Humans Spectrophotometry Ultraviolet gamma-Globulins Protein Binding |
| Zdroj: | Bulletin of experimental biology and medicine. 142(6) |
| ISSN: | 0007-4888 |
| Popis: | Specimens of human serum gamma-globulin modified by molar excess of copper and zinc cations were obtained by molecular ultrafiltration. Conformation characteristics of the protein were determined by UV spectrophotometry. Immunochemical study included radial immunodiffusion test and direct and sandwich enzyme-linked immunosorbent assay. After binding of copper and zinc, the gamma-globulin molecule underwent conformation changes modifying presentation of antigenic determinants on the globule surface and their availability for recognition by specific antibodies. The effects of copper were much more pronounced than those of zinc cations. |
| Databáze: | OpenAIRE |
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