Sex-linked differences in the vasorelaxant effects of anandamide in vascular mesenteric beds: role of oestrogens

Autor: Roxana N. Peroni, Damasia Becu-Villalobos, Stella Maris Celuch, Juan Pablo Huidobro-Toro, Edda Adler-Graschinsky, Marı́a Luz Orliac
Rok vydání: 2004
Předmět:
Male
Time Factors
VASORELAXATION
medicine.medical_treatment
Muscle
Smooth
Vascular
Rats
Sprague-Dawley
Norepinephrine
chemistry.chemical_compound
Piperidines
Mesentery
Chile
Cycloheximide
Sex Characteristics
Estradiol
Drug Synergism
Patología
Anandamide
Bioquímica y Biología Molecular
Receptor antagonist
Vasodilation
Medicina Básica
Female
Rimonabant
Capsazepine
Nitroprusside
Agonist
SEX DIFFERENCE
medicine.medical_specialty
CIENCIAS MÉDICAS Y DE LA SALUD
Polyunsaturated Alkamides
medicine.drug_class
Ovariectomy
Argentina
TRPV1
Arachidonic Acids
Biology
Internal medicine
medicine
Animals
Pharmacology
Dose-Response Relationship
Drug
Estrogens
Acetylcholine
Rats
Phenylmethylsulfonyl Fluoride
ANANDAMIDE
Endocrinology
chemistry
17 BETA OESTRADIOL
Capsaicin
Pyrazoles
Cannabinoid receptor antagonist
Cannabinoid
Endocannabinoids
Zdroj: European Journal of Pharmacology. 493:151-160
ISSN: 0014-2999
DOI: 10.1016/j.ejphar.2004.04.031
Popis: Anandamide (0.01 to 10 microM) caused greater concentration-dependent reductions of the contractile-induced responses to noradrenaline in female than in male mesenteric vascular beds isolated from adult Sprague-Dawley rats. Greater relaxant responses in females were also induced by the vanilloid TRPV1 receptor agonist capsaicin (0.01 to 10 microM), whereas no sex differences were observed for the relaxations caused by either acetylcholine or sodium nitroprusside. The effect of anandamide in either sex was reduced by the vanilloid TRPV1 receptor antagonist capsazepine but not by the cannabinoid CB1 receptor antagonist N-piperidino-5-(4-chlorophenyl)-1-(2,4-dichlorophenyl)-4-methyl-3-pyrazole-carboxamide (SR141716A). In males, the anandamide-induced relaxations were potentiated by in vitro exposure during 5 min to 0.5 microM 17beta-oestradiol and unmodified by the protein synthesis inhibitor cycloheximide. The vasorelaxant effects of anandamide in female rats were decreased by ovariectomy. This decrease was prevented by in vivo treatment with 17beta-oestradiol-3-benzoate (450 microg/kg i.m., once a week during 3 weeks) and counteracted by in vitro exposure to oestrogen. In vivo treatment with 17beta-oestradiol also potentiated anandamide-induced responses in males. In conclusion, this study shows an oestrogen-dependent sensitivity to the vanilloid TRPV1 receptor-mediated vasorelaxant effects of anandamide in the mesenteric vasculature of Sprague-Dawley rats, that could be mediated by both genomic and non-genomic mechanisms Fil: Peroni, Roxana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Orliac, Maria Luz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Becu, Damasia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina Fil: Huidobro Toro, Juan Pablo. Pontificia Universidad Católica de Chile; Chile Fil: Adler, Edda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina Fil: Celuch, Stella Maris. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Investigaciones Farmacológicas; Argentina
Databáze: OpenAIRE
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