Gonadotoxic effects of busulfan in two strains of mice
| Autor: | Karina Gutierrez, Werner G. Glanzner, Raiza O. Chemeris, Vilceu Bordignon, Paulo Bayard Dias Gonçalves, Fabio V. Comim, Melânia L. Rigo |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Kruppel-Like Transcription Factors Toxicology Busulfan Injection Andrology 03 medical and health sciences Species Specificity Pregnancy Testis medicine Glial cell line-derived neurotrophic factor Animals Promyelocytic Leukemia Zinc Finger Protein Glial Cell Line-Derived Neurotrophic Factor Antineoplastic Agents Alkylating Busulfan Cell Shape Infertility Male Mice Inbred BALB C biology business.industry Gene Expression Regulation Developmental RNA-Binding Proteins Spermatozoa Testicular degeneration Fertility 030104 developmental biology Chemotherapy Drugs Immunology Sperm Motility Sperm morphology biology.protein Female Histopathology business Live Birth After treatment medicine.drug |
| Zdroj: | Reproductive Toxicology. 59:31-39 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2015.09.002 |
| Popis: | Busulfan is a chemotherapy drug that has side effects on spermatogonial stem cells (SSC). The effects of bulsufan treatment on male germ cells and fertility vary significantly between individuals. In this study, we have used molecular, cellular and histopathology approaches to investigate the effects of a single intraperitoneal dose of busulfan (40 mg kg −1 ) in two mice strains, Balb/C and Swiss, at two different periods after treatment, 30 and 90 days. Testicular degeneration was observed in both Balb/C and Swiss mice after busulfan injection. Interestingly, testicular functions and fertility recovered spontaneously post busulfan treatment in Swiss mice, but not in Balb/C mice. Abnormal fertility induced by busulfan in Balb/C mice was associated with altered seminiferous tubules, sperm morphology and transcript levels of Nanos2, Nanos3 , Gdnf and Plzf genes. These findings revealed that SSC of Balb/C mice are more sensitive to the toxic effects of busulfan then those of Swiss mice. |
| Databáze: | OpenAIRE |
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