A preliminary study on the impact of exogenous A-Factor analogue 1,4-butyrolactone on stimulating bitespiramycin biosynthesis
| Autor: | Yonghong Wang, Xiaowei Gao, Ju Chu |
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| Rok vydání: | 2019 |
| Předmět: |
Time Factors
Bitespiramycin Bioengineering Microbial Sensitivity Tests Catalysis chemistry.chemical_compound Industrial Microbiology Biosynthesis 4-Butyrolactone Spiramycin medicine Chromatography High Pressure Liquid chemistry.chemical_classification Chemistry General Medicine Streptomyces Anti-Bacterial Agents Titer Enzyme Biochemistry Fermentation Industrial and production engineering Biotechnology medicine.drug Signal Transduction |
| Zdroj: | Bioprocess and biosystems engineering. 42(12) |
| ISSN: | 1615-7605 |
| Popis: | Bitespiramycin is composed of nine main acylated spiramycin components with isovaleryspiramycin as the major component. However, even with excellent therapeutic effects, its application and industrialization are restricted due to its low titer. In this study, the exogenous addition of A-Factor analogue 1,4-butyrolactone (1,4-BL) stimulated an improvement in bitespiramycin biological titer by 29% with a tiny influence on concentration of major component. Moreover, the mechanism of 1,4-BL stimulating effect was preliminarily explored by the analyses of three key enzyme activities, intracellular metabolite profiling and metabolic flux distribution. All results coordinately revealed that the extensive accumulation of methylmalonyl-CoA and acetyl-CoA was the direct reason for the enhanced bitespiramycin biosynthesis. This study would provide theoretical and technical basis for the application of 1,4-BL addition strategy to industrial bitespiramycin production. |
| Databáze: | OpenAIRE |
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