Actin-Binding Proteins from Burkholderia mallei and Burkholderia thailandensis Can Functionally Compensate for the Actin-Based Motility Defect of a Burkholderia pseudomallei bimA Mutant
| Autor: | Michael W. Wood, Ricky L. Ulrich, Joanne M. Stevens, Edouard E. Galyov, David DeShazer, Lowrie A. Taylor, Mark P. Stevens |
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| Rok vydání: | 2005 |
| Předmět: |
Burkholderia pseudomallei
Burkholderia Immunoblotting Molecular Sequence Data Motility macromolecular substances Burkholderia mallei Microbiology Cell Line Mice Bacterial Proteins Animals Amino Acid Sequence Actin-binding protein Molecular Biology Actin Molecular Biology of Pathogens Microscopy Confocal Sequence Homology Amino Acid biology Burkholderia thailandensis Macrophages Genetic Complementation Test Microfilament Proteins bacterial infections and mycoses biology.organism_classification Actin-Related Protein 3 Mutation biology.protein bacteria Gene Deletion Protein Binding |
| Zdroj: | Stevens, J M, Ulrich, R L, Taylor, L A, Wood, M W, DeShazer, D, Stevens, M P & Galyov, E E 2005, ' Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant ', Journal of Bacteriology, vol. 187, no. 22, pp. 7857-7862 . https://doi.org/10.1128/JB.187.22.7857-7862.2005 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei . Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defect of a B. pseudomallei bimA mutant. While the BimA homologs differ in their amino-terminal sequence, they interact directly with actin in vitro and vary in their ability to bind Arp3. |
| Databáze: | OpenAIRE |
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