Comparison of PCR and antigen detection methods for diagnosis of Entamoeba histolytica infection
| Autor: | P L Chiodini, S J Furrows, A H Moody |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Antigens
Protozoan Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Pathology and Forensic Medicine law.invention Feces Entamoeba histolytica fluids and secretions Antigen law parasitic diseases Entamoeba histolytica Infection Animals Humans Protozoal disease Polymerase chain reaction Laboratory methods Entamoebiasis biology Nucleic Acid Hybridization Original Articles General Medicine DNA Protozoan biology.organism_classification Virology Molecular biology digestive system diseases Enzyme linked immunoassay |
| Zdroj: | Journal of Clinical Pathology. 57:1264-1266 |
| ISSN: | 0021-9746 |
| DOI: | 10.1136/jcp.2004.017822 |
| Popis: | Aims: To assess different laboratory methods for the identification of Entamoeba histolytica in clinical samples. Methods: Antigen detection enzyme linked immunosorbent assay, polymerase chain reaction solution hybridisation enzyme linked immunoassay (PCR-SHELA), and a commercial Lightcycler PCR were compared using 101 stool and pus samples. Results: Fifteen of the 101 samples were positive for E histolytica by one or more method. There were discrepancies between the results in five of these 15 samples when the assays were compared. Conclusions: All three methods performed adequately, so that the choice of assay will depend on each individual laboratory’s budget and projected turnaround time. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|