Molecular Characterization of pssCDE Genes of Rhizobium leguminosarum bv. trifolii strain TA1: pssD Mutant Is Affected in Exopolysaccharide Synthesis and Endocytosis of Bacteria
| Autor: | Wladyslaw Golinowski, Andrzej Mazur, Barbara Lotocka, Jerzy Wielbo, Anna Skorupska, Joanna Kopcińska, Jarosław E. Król |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Rhizobium leguminosarum
Rhizobiaceae Physiology Molecular Sequence Data Mutant General Medicine Biology Poaceae Endocytosis medicine.disease_cause biology.organism_classification Phenotype Homology (biology) Microbiology Microscopy Electron Genes Bacterial Polysaccharides Nitrogen Fixation Mutation medicine Agronomy and Crop Science Gene Bacteria |
| Zdroj: | Molecular Plant-Microbe Interactions®. 11:1142-1148 |
| ISSN: | 1943-7706 0894-0282 |
| Popis: | We have identified the three genes pssCDE in Rhizobium leguminosarum bv. trifolii TA1. Even though they were almost identical to earlier identified pssCDE genes of R. leguminosarum, they differed in gene lengths and gene overlaps. The predicted gene products of pssCDE genes shared significant homology to prokaryotic glycosyl transferases involved in exopolysaccharide synthesis. The Tn5 insertion in pssD created the nonmucoid mutant that induced non-nitrogen-fixing nodules. The microscopic analysis of the nodules, induced on Trifolium pratense by the pssD133 mutant, showed abnormally enlarged infection threads densely packed with bacteria, which were released from the infection threads in an unusual way. The symbiosomes were observed very rarely and the nodule remained almost empty. Symbiotic phenotype of the pssD133 suggested a correlation between this mutation and defective endocytosis of bacteria into nodule cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|