Molecular identification of coccidioides isolates from Mexican patients

Autor:	Diego Güereña-Elizalde, Arturo Aroch-Calderón, Alexei Fedorovich Licea-Navarro, Laura Rocío Castañón-Olivares, Marisela del Rocío González-Martínez, Gloria María González-González
Rok vydání:	2007
Předmět:	Microbiological Techniques Coccidioides immitis Single-nucleotide polymorphism Fungus Genome Polymorphism Single Nucleotide General Biochemistry Genetics and Molecular Biology History and Philosophy of Science Species Specificity SNP Humans Coccidioides DNA Fungal Mycological Typing Techniques Mexico DNA Primers Genetics Coccidioidomycosis biology Geography General Neuroscience Sputum biology.organism_classification Coccidioides posadasii Chemistry Clinical SNP array
Zdroj:	Annals of the New York Academy of Sciences. 1111
ISSN:	0077-8923
Popis:	Molecular studies of the genome of the fungus Coccidioides have demonstrated two nearly identical, but well-identified species, Coccidioides immitis and C. posadasii, known as “California” and “non-California” species, respectively. The objective of this study was to determine, through molecular methods, whether both species of Coccidioides are present in Mexican patients with coccidioidomycosis and to estimate, their geographical distribution in Mexico. We analyzed 56 clinical isolates of Coccidioides spp. from Mexican patients. Molecular identification of each strain was done by means of real time PCR using TaqMan® probes to amplify single nucleotide polymorphisms (SNPs) in four target sequences, loci, named proline 157, proline 174, hexokinase 149 and glucose-synthase 192. SNP analysis identified two of the 56 isolates as Coccidioides immitis and the remaining 54 as C. posadasii. The dual probe assay that included proline 157, proline 174 and glucose-synthase 192 gave consistent results on SNP differentiation between the two species. In contrast, the template matching hexokinase 149 gave negative results for any species in 34 samples. Our results did not show geographical overlap of the species, and they also confirmed that C. posadasii is the most frequent species in Mexico. A vast majority of C. posadasii strains were localized in the north-central region of the country.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f258649aa5bb12b4947d9f32c08dc3ec https://pubmed.ncbi.nlm.nih.gov/17344538 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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