Synthesis of a 3'-naphthamido-LacNAc fluorescein conjugate with high selectivity and affinity for galectin-3
| ISSN: | 0008-6215 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f25777cb5f433235dcca78e1f9fe5ef0 https://pubmed.ncbi.nlm.nih.gov/16697983 |
| Rights: | CLOSED |
| Přírůstkové číslo: | edsair.doi.dedup.....f25777cb5f433235dcca78e1f9fe5ef0 |
| Autor: | Ulf J. Nilsson, Jörgen Toftered, Hakon Leffler, Anders Sundin, Karolina Aplander, Barbro Kahl-Knutsson, Susanne Carlsson, Johan Tejler |
| Rok vydání: | 2006 |
| Předmět: |
Chromatography
biology Chemistry Galectin 3 Organic Chemistry Stacking Lectin Fluorescence Polarization General Medicine Disaccharides Fluoresceins Biochemistry Combinatorial chemistry Fluorescence Analytical Chemistry chemistry.chemical_compound biology.protein Fluorescein Selectivity Fluorescence anisotropy Galectin Conjugate |
| Zdroj: | Carbohydrate research. 341(10) |
| ISSN: | 0008-6215 |
| Popis: | Described is the synthesis of a fluorescent LacNAc derivative appended with a 3'-deoxy-3'-naphthamido functionality, 2-(fluorescein-5/6-amido)ethyl 3-deoxy-3-(2-naphthamido)-beta-D-galactopyranosyl-(1-->4)-2-acetamido-2-deoxy-beta-D-glucopyranoside, which confers high affinity (Kd 170 nM) and selectivity for galectin-3 via a stacking interaction with Arg144. Its use as a selective and sensitive galectin-3 probe is demonstrated with fluorescence polarization measurements. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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