Expression and correlation of NRF2, KEAP1, NQO-1 and HO-1 in advanced squamous cell carcinoma of the larynx and their association with clinicopathologic features
| Autor: | Haitao Wu, Shuyi Wang, Jiaxing Zhu, Changjiang Li |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Pathology medicine.medical_specialty NF-E2-Related Factor 2 medicine.medical_treatment HO-1 Gene Expression Biology Biochemistry NRF2 03 medical and health sciences 0302 clinical medicine Gene expression Genetics Carcinoma medicine Biomarkers Tumor NAD(P)H Dehydrogenase (Quinone) Humans Molecular Biology Laryngeal Neoplasms Aged Neoplasm Staging Aged 80 and over Kelch-Like ECH-Associated Protein 1 Oncogene Cancer Articles Laryngeal Neoplasm Middle Aged medicine.disease Prognosis Molecular medicine Immunohistochemistry Laryngectomy advanced laryngeal squamous cell carcinoma KEAP1 030104 developmental biology Oncology 030220 oncology & carcinogenesis Carcinoma Squamous Cell Molecular Medicine NQO-1 Female Heme Oxygenase-1 |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | The present study aimed specifically investigate the expression and correlation of kelch-like ECH-associated protein‑1 (KEAP1), nuclear factor (erythroid-derived 2)‑like 2 (NRF2), quinone oxidoreductase‑1 (NQO‑1) and heme oxygenase‑1 (HO‑1) in laryngeal cancer and their association with clinicopathological features. A total of 33 paired human fresh advanced laryngeal cancer and adjacent normal specimens were collected following total laryngectomy. Immunohistochemical and immunoblotting analysis were performed to evaluate the expression of KEAP1, NRF2, NQO‑1 and HO‑1. All of the patients clinicopathologic features were collected. Immunohistochemistry indicated that NRF2‑positive staining was as high as 79% (26/33) in the cancer samples and predominantly located in the nuclei, whereas positive expression of KEAP1, NQO‑1 and HO‑1 was detected in 70% (23/33), 76% (25/33) and 82% (27/33) of cancer tissues, respectively, and primarily expressed in the cytoplasm. The corresponding adjacent normal samples produced almost no or weak expression of the proteins. There were significant differences in protein expression between the two groups (P |
| Databáze: | OpenAIRE |
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