The ability of cyanobacterial cells to restore UV-B radiation induced damage to Photosystem II is influenced by photolyase dependent DNA repair
| Autor: | Imre Vass, István-Zoltán Vass, Csaba István Nagy, László Sass, Péter B. Kós |
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| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial DNA Repair Transcription Genetic DNA damage DNA repair Ultraviolet Rays Mutant macromolecular substances Biology Biochemistry chemistry.chemical_compound Bacterial Proteins Transcription (biology) Protein Isoforms Physical and Theoretical Chemistry Photosynthesis Photolyase Gene Wild type Synechocystis Photosystem II Protein Complex General Medicine Molecular biology chemistry Protein Biosynthesis Deoxyribodipyrimidine Photo-Lyase DNA DNA Damage |
| Zdroj: | Photochemistry and photobiology. 89(2) |
| ISSN: | 1751-1097 |
| Popis: | Damage of DNA and Photosystem-II are among the most significant effects of UV-B irradiation in photosynthetic organisms. Both damaged DNA and Photosystem-II can be repaired, which represent important defense mechanisms against detrimental UV-B effects. Correlation of Photosystem-II damage and repair with the concurrent DNA damage and repair was investigated in the cyanobacterium Synechocystis PCC6803 using its wild type and a photolyase deficient mutant, which is unable to repair UV-B induced DNA damages. A significant amount of damaged DNA accumulated during UV-B exposure in the photolyase mutant concomitant with decreased Photosystem-II activity and D1 protein amount. The transcript level of psbA3, which is a UV-responsive copy of the psbA gene family encoding the D1 subunit of the Photosystem-II reaction center, is also decreased in the photolyase mutant. The wild-type cells, however, did not accumulate damaged DNA during UV-B exposure, suffered smaller losses of Photosystem-II activity and D1 protein, and maintained higher level of psbA3 transcripts than the photolyase mutant. It is concluded that the repair capacity of Photosystem-II depends on the ability of cells to repair UV-B-damaged DNA through maintaining the transcription of genes, which are essential for protein synthesis-dependent repair of the Photosystem-II reaction center. |
| Databáze: | OpenAIRE |
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