Manipulation of the DNA coding for the desulphurizing activity in a new isolate of Arthrobacter sp
| Autor: | F. de Ferra, Serbolisca Luca Paolo, I. Margarit |
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| Rok vydání: | 1999 |
| Předmět: |
DNA
Bacterial Operon Cloning vector Thiophenes Applied Microbiology and Biotechnology law.invention chemistry.chemical_compound Plasmid law Arthrobacter Escherichia coli Cloning biology General Medicine biology.organism_classification Electrophoresis Gel Pulsed-Field chemistry Biochemistry Genes Bacterial Recombinant DNA Transformation Bacterial Oxidoreductases Sulfur Bacteria DNA Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 52:122-126 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s002530051498 |
| Popis: | A new bacterial strain able to cleave C-S bonds from organosulphur heterocyclic compounds through the 4-S pathway and tentatively classified as Arthrobacter sp. was recently isolated. In the present short article we describe the cloning and the characterization of the DNA encoding the enzymes responsible for desulphurization in this microorganism, referred to as Arthrobacter sp. DS7. The desulphurization operon was found to be located in a large plasmid that also bears the genes conferring cadmium and arsenic resistance. By shortening this plasmid, a new cloning vector was prepared and used to obtain a recombinant derivative strain that desulphurizes dibenzothiophene despite of the presence of inorganic sulphur in the growth medium. |
| Databáze: | OpenAIRE |
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