Molecular cloning and allergenicity of Pen j 1, a major allergen of kuruma prawn, Penaeus japonicus
| Autor: | Hideaki Tsuji, Tomoaki Matsumoto, Takako Sisino, Kyoko Takahashi, Miki Hiemori, Masumi Kimoto, Ayumi Kunimoto, Hiromi Yamashita, Kentaro Sakai |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male DNA Complementary Adolescent Sequence analysis Molecular Sequence Data Tropomyosin Biology Molecular cloning medicine.disease_cause Applied Microbiology and Biotechnology Biochemistry Epitope Analytical Chemistry Blood serum Allergen Penaeidae Complementary DNA medicine Escherichia coli Animals Humans Amino Acid Sequence Cloning Molecular Child Molecular Biology Peptide sequence Base Sequence Organic Chemistry Infant General Medicine Sequence Analysis DNA Allergens Immunoglobulin E Middle Aged Fusion protein Molecular biology Peptide Fragments Child Preschool Female Food Hypersensitivity Biotechnology |
| Zdroj: | Bioscience, biotechnology, and biochemistry. 73(4) |
| ISSN: | 1347-6947 |
| Popis: | Tropomyosins have been identified as a common allergen in crustaceans, but their allergenicity is not well understood. In the present study, we isolated an allergen, Pen j 1, a tropomyosin from kuruma prawn Penaeus japonicus, and determined its N-terminal amino acid sequence. The cDNA encoding the allergen was cloned by 5'- and 3'-rapid amplification of cDNA ends (RACE), and was found to code for a protein which consists of 284 amino acid residues. Sequencing analyses indicated for the first time that mature tropomyosin is formed by the elimination of a leader peptide of nine amino acid residues. To elucidate the binding sites of IgE antibodies in the sera of shrimp-sensitive patients, various recombinant peptides were expressed in Escherichia coli as fusion proteins with glutathione S-transferase (GST), and the examined with regard to reactivity with IgE antibodies. The IgE-binding epitopes were found to locate over the whole sequence of the allergen, and the IgE antibodies in the sera were found to recognize strongly its C-terminal region. |
| Databáze: | OpenAIRE |
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