Diesel2p mesoscope with dual independent scan engines for flexible capture of dynamics in distributed neural circuitry
| Autor: | Riichiro Hira, Che-Hang Yu, Jeffrey N. Stirman, Yiyi Yu, Spencer L. Smith |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Microscope
Computer science Science General Physics and Astronomy Bioengineering Temporal multiplexing Neural circuits Fluorescence Article Fluorescence imaging General Biochemistry Genetics and Molecular Biology Multiphoton microscopy law.invention Calcium imaging law Biological neural network Premovement neuronal activity Computer vision Adaptive optics Multiphoton Neurons Flexibility (engineering) Microscopy Multidisciplinary business.industry Optical Imaging Neurosciences Brain Equipment Design General Chemistry DUAL (cognitive architecture) Microscopy Fluorescence Multiphoton Neurological Biomedical Imaging Calcium Artificial intelligence Nerve Net business Computer hardware |
| Zdroj: | Nature Communications, Vol 12, Iss 1, Pp 1-8 (2021) Nature Communications Nature communications, vol 12, iss 1 |
| ISSN: | 2041-1723 |
| Popis: | Imaging the activity of neurons that are widely distributed across brain regions deep in scattering tissue at high speed remains challenging. Here, we introduce an open-source system with Dual Independent Enhanced Scan Engines for Large field-of-view Two-Photon imaging (Diesel2p). Combining optical design, adaptive optics, and temporal multiplexing, the system offers subcellular resolution over a large field-of-view of ~25 mm2, encompassing distances up to 7 mm, with independent scan engines. We demonstrate the flexibility and various use cases of this system for calcium imaging of neurons in the living brain. Imaging of neuronal activity across distant brain regions is challenging. Here, the authors introduce a two-photon microscope with two independently controlled scan engines, and demonstrate calcium imaging with subcellular resolution in brain regions up to 7 mm apart simultaneously. |
| Databáze: | OpenAIRE |
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