Expression of feline immunodeficiency virus Vif is associated with reduced viral mutation rates without restoration of replication of vif mutant viruses
| Autor: | Thomas A. Paul, Roger J. Avery, Claudia A. Sutton, James W. Casey |
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| Rok vydání: | 2007 |
| Předmět: |
Cytoplasm
Feline immunodeficiency virus Gene Products vif Virus mutation viruses Molecular Sequence Data Mutant FIV-Oma Immunodeficiency Virus Feline Transfection Virus Replication Virus Cell Line 03 medical and health sciences Retrovirus Feline Acquired Immunodeficiency Syndrome Virology Animals Amino Acid Sequence Codon Gene APOBEC3G Cellular localization 030304 developmental biology Genetics 0303 health sciences Base Sequence biology 030302 biochemistry & molecular biology Lentivirus virus diseases biochemical phenomena metabolism and nutrition biology.organism_classification FIV Vif 3. Good health Viral replication Cats |
| Zdroj: | Virology. 361(1):112-122 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2006.11.005 |
| Popis: | The vif gene of lentiviruses has been demonstrated to be essential for efficient viral replication in many cell types. Although the Vif protein of feline immunodeficiency virus (FIV) displays limited homology to HIV-1 Vif, the role of vif in FIV replication is not known. We have examined the requirements of vif for replication of a FIV strain isolated from a non-domestic felid, Otocolobus manul (FIV-Oma). In agreement with others, we find that replication of FIV vif mutant molecular clones in CrFK cells is highly attenuated. Initial attempts to rescue vif mutant viruses in trans were limited by lack of detectable wild-type Vif expression from DNA constructs. We demonstrate that FIV-Oma Vif expression can be increased by re-synthesis of the gene to remove splice donor and acceptor sites as well as improving codon usage to a mammalian codon optimized model. Cellular localization of resynthesized Vif (Vif-RS) is cytoplasmic. Clonal stable transfectants expressing HA-tagged Vif-RS do not restore replication levels of vif mutant virus. However, in such cell lines, G-to-A mutation rates in replicating wild-type viruses are reduced. |
| Databáze: | OpenAIRE |
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