Subfractionation of rat liver microsomes: Effects of phenobarbital and 3-methyl cholanthrene
| Autor: | P.J. Murphy, T.L. Williams, R. M. Van Frank |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Male
Cytochrome Biophysics Biochemistry chemistry.chemical_compound medicine Animals Distribution (pharmacology) Centrifugation Ethionine Molecular Biology biology Chemistry Vesicle Cell Biology Amides Centrifugation Zonal Rats Enzyme Induction Phenobarbital Microsomes Liver biology.protein Microsome Cytochromes Thioacetamide Densitometry Methylcholanthrene medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 37:697-704 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(69)90867-5 |
| Popis: | Subfractionation of rat liver microsomes by the use of rate-zonal centrifugation has been carried out using both normal and induced rats. Phenobarbital and methyl cholanthrene were found to have very distinctive effects on the distribution of cytochrome P-450 within the microsomal vesicles. The altered distribution observed with methyl cholanthrene is inhibited by thioacetamide and ethionine. These results suggest that the P-450 produced after treatment with methyl cholanthrene is preferentially formed in, or bound to, a vesicle distinct from that which contains the P-450 in the normal or phenobarbital-induced animal. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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