Simultaneous detection of 9 respiratory pathogens using a newly developed multiplex real-time PCR panel based on an automatic molecular detection and analysis system

Autor:	Meng-chuan Zhao, Yue Jiang, Gui-xia Li, Yan-qing Tie, Ye-huan Zheng, Jin-fu Li, Wen-chao Zhang, Su-xia Duan, Yu Zhai, Yuan-long Li, Di-jun Zhang, Xian-ping Zeng, Yong Wu, Ying-hui Guo, Zhi-shan Feng
Rok vydání:	2022
Předmět:	Microbiology (medical) Infectious Diseases Respiratory Syncytial Virus Human Humans General Medicine Metapneumovirus Real-Time Polymerase Chain Reaction Multiplex Polymerase Chain Reaction Respiratory Tract Infections Sensitivity and Specificity
Zdroj:	Diagnostic microbiology and infectious disease. 104(4)
ISSN:	1879-0070
Popis:	Timely identification of respiratory pathogens guides specific treatment, reduces hospital costs and minimizes the excessive use of antibiotics. A new multiplex real-time PCR panel was developed based on an automatic molecular detection and analysis system (AutoMolec system), consisting of three separate internally controlled assays. Mycoplasma pneumoniae, Chlamydia pneumoniae, adenovirus, human metapneumovirus, influenza B virus, respiratory syncytial virus and human parainfluenza virus 1-3 may be directly detected in original samples. The system's clinical performance was evaluated by comparison with an approved commercial kit, using 517 clinical samples. The limit of detection of the AutoMolec mRT-PCR panel ranged from 4 × 10
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2499d1fa2188f5ebe7e8051e5a1e21f https://pubmed.ncbi.nlm.nih.gov/36130430 Zobrazit plný text záznamu Full Text from ScienceDirect