Flutamide treatment reveals a relationship between steroidogenic activity of Leydig cells and ultrastructure of their mitochondria
| Autor: | Waclaw Tworzydlo, Anna Hejmej, Szczepan M. Bilinski, Barbara Bilińska, Sylwia Marek, Malgorzata Brzoskwinia, Laura Pardyak, Alicja Kamińska |
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| Rok vydání: | 2021 |
| Předmět: |
Male
0301 basic medicine Cell biology endocrine system Physiology Science MFN2 Mitochondrion Article Flutamide 03 medical and health sciences chemistry.chemical_compound Endocrinology Aromatase 0302 clinical medicine Testis medicine Animals Testosterone Cholesterol Side-Chain Cleavage Enzyme Gonadal Steroid Hormones 030219 obstetrics & reproductive medicine Multidisciplinary Leydig cell Chemistry Cholesterol side-chain cleavage enzyme Hydroxysteroid Dehydrogenases Gene Expression Regulation Developmental Leydig Cells Luteinizing Hormone Mitochondria Rats Androgen receptor 030104 developmental biology medicine.anatomical_structure Receptors Androgen Sex steroid Medicine Steroids |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-021-93292-8 |
| Popis: | Our present knowledge on interrelation between morphology/ultrastructure of mitochondria of the Leydig cell and its steroidogenic function is far from satisfactory and needs additional studies. Here, we analyzed the effects of blockade of androgen receptor, triggered by exposure to flutamide, on the expression of steroidogenic proteins (1) and ultrastructure of Leydig cells’ constituents (2). We demonstrated that increase in the expression level of steroidogenic (StAR, CYP11A1, 3β-HSD, and CYP19A1) proteins (and respective mRNAs) in rat testicular tissue as well as elevation of intratesticular sex steroid hormone (testosterone and estradiol) levels observed in treated animals correspond well to morphological alterations of the Leydig cell ultrastructure. Most importantly, up-regulation of steroidogenic proteins’ expression apparently correlates with considerable multiplication of Leydig cell mitochondria and subsequent formation of local mitochondrial networks. Interestingly, we showed also that the above-mentioned processes were associated with elevated transcription of Drp1 and Mfn2 genes, encoding proteins implicated in mitochondrial dynamics. Collectively, our studies emphasize the importance of mitochondrial homeostasis to the steroidogenic function of Leydig cells. |
| Databáze: | OpenAIRE |
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