Peri-tunnel bone loss: does it affect early tendon graft to bone tunnel healing after ACL reconstruction?
| Autor: | Tsui Yu Mok, Pauline Po Yee Lui, Yuk Wa Lee, Yau Chuk Cheuk |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Bone density Peri Rat model Antigens Differentiation Myelomonocytic Cruciate ligament Rats Sprague-Dawley Tendons Antigens CD Bone Density Matrix Metalloproteinase 13 medicine Animals Orthopedics and Sports Medicine Femur Tendon graft Wound Healing Anterior Cruciate Ligament Reconstruction Tibia business.industry Anterior Cruciate Ligament Injuries Immunohistochemistry Surgery medicine.anatomical_structure Orthopedic surgery Bone tunnel Matrix Metalloproteinase 1 business Tomography X-Ray Computed Epiphyses |
| Zdroj: | Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA. 23(3) |
| ISSN: | 1433-7347 |
| Popis: | The clinical relevance and mechanisms of local bone loss early post-anterior cruciate ligament (ACL) reconstruction remain unclear. The early spatial and temporal changes of peri-tunnel bone, its molecular mechanisms and its relationships with graft-bone tunnel healing were investigated in a 12-week-old rat model.At various times, the reconstructed ACL complex was harvested for vivaCT imaging, biomechanical test, histology and immunohistochemical staining of CD68+ cells (a monocyte-macrophage lineage marker), MMP1 and MMP13.The peri-tunnel bone resorbed simultaneously with improvement of graft-bone tunnel healing. There were 30.1 ± 17.4, 46.8 ± 10.5 and 81.5 ± 12.3 % loss of peri-tunnel BMD as well as 43.2 ± 21.7, 78.7 ± 8.5 and 92.4 ± 17.7 % loss of peri-tunnel bone volume/total volume (BV/TV) at week 6 at the distal femur, epiphysis and metaphysis of tibia, respectively. MMP1, MMP13 and CD68+ cells were expressed at the graft-bone tunnel interface and peri-tunnel bone and increased with time post-reconstruction at the tibia. The ultimate load and stiffness of the healing complex positively correlated with tibial tunnel bone formation and negatively correlated with tibial peri-tunnel bone. Tunnel BV/TV at the tibial metaphysis and epiphysis showed the highest correlation with ultimate load (ρ = 0.591; p = 0.001) and stiffness (ρ = 0.427; p = 0.026) of the complex, respectively.There was time-dependent loss of peri-tunnel bone early post-reconstruction, with the greatest loss occurring at the tibial metaphysis. This was consistent with high expression of MMP1, MMP13 and CD68+ cells at the graft-bone tunnel interface and the peri-tunnel region. The significant loss of peri-tunnel bone, though not critically affecting early tunnel healing, suggested the need to protect the knee joint early post-reconstruction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink