Plant-produced Hepatitis B Core Protein Chimera Carrying Anthrax Protective Antigen Domain-4
| Autor: | Sergei Spitsin, Natalia Pogrebnyak, Thomas O. Kohl, Hilary Koprowski, Robert Brodzik, Maxim Golovkin, Katarzyna Bandurska, Carla Portocarrero, Yuri Smirnov, Agnieszka Sirko |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.drug_class
Recombinant Fusion Proteins Bacterial Toxins Immunology Anthrax Vaccines Monoclonal antibody Models Biological Epitope Mice Immune system Antigen Tobacco medicine Animals Immunology and Allergy Antigens Bacterial Mice Inbred BALB C Anthrax vaccines biology fungi food and beverages Plants Genetically Modified biology.organism_classification Hepatitis B Core Antigens Virology Protein Structure Tertiary Bacillus anthracis HBcAg Macrophage-1 antigen Female |
| Zdroj: | Hybridoma. 27:241-247 |
| ISSN: | 1557-8348 1554-0014 |
| DOI: | 10.1089/hyb.2008.0008 |
| Popis: | The hepatitis B core antigen (HBcAg) can generate a strong immune response and is recognized as an effective carrier for foreign epitopes. The domain-4 epitope of the anthrax protective antigen (PA-D4) plays an essential role in generating protective immunity against virulent Bacillus anthracis. Here we report the successful production of a recombinant protein comprised of the antigenic PA-D4 integrated into the c/e1 loop of HBcAg in transgenic low-alkaloid Nicotiana tabacum. Sera of mice injected with the plant-derived purified HB/PA-D4 protein exhibited significant anti-PA- and anti-HBcAg-specific IgG titers; however, formation of virus-like particles (VLP) was not observed. These data support the feasibility of producing complex protein chimeras in plants. |
| Databáze: | OpenAIRE |
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