Prime-boost vaccination with plasmid DNA and a chimeric adenovirus type 5 vector with type 35 fiber induces protective immunity against HIV
| Autor: | K-Q Xin, N Jounai, K Someya, K Honma, H Mizuguchi, S Naganawa, K Kitamura, T Hayakawa, S Saha, F Takeshita, K Okuda, M Honda, D M Klinman |
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| Rok vydání: | 2005 |
| Předmět: |
Male
viruses Genetic enhancement Genetic Vectors Immunization Secondary HIV Infections Vaccinia virus Biology Antibodies Viral medicine.disease_cause Virus Adenoviridae Viral vector Mice Viral Proteins chemistry.chemical_compound Neutralization Tests Vaccines DNA Genetics medicine Animals HIV vaccine Molecular Biology AIDS Vaccines Mice Inbred BALB C Immunogenicity virus diseases Genetic Therapy biochemical phenomena metabolism and nutrition Macaca mulatta Virology Vaccination chemistry DNA Viral Models Animal Immunology HIV-1 Molecular Medicine Female Immunization Vaccinia Virus Physiological Phenomena |
| Zdroj: | Gene Therapy. 12:1769-1777 |
| ISSN: | 1476-5462 0969-7128 |
| Popis: | Immunization involving a DNA vaccine prime followed by an adenovirus type 5 (Ad5) boost elicited a protective immune response against SHIV challenge in monkeys. However, the hepatocellular tropism of Ad5 limits the safety of this viral vector. This study examines the safety and immunogenicity of a replication-defective chimeric Ad5 vector with the Ad35 fiber (Ad5/35) in BALB/c mice and rhesus monkeys. This novel Ad5/35 vector showed minimal hepatotoxicity after intramuscular administration with the novel Ad5/35 vector. In addition, an Ad5/35 vector expressing HIV Env gp160 protein (Ad5/35-HIV) generated strong HIV-specific immune responses in both animal models. Priming with a DNA vaccine followed by Ad5/35-HIV boosting yielded protection against a gp160-expressing vaccinia virus challenge in BALB/c mice. The Ad5/35-HIV vector was significantly less susceptible to the pre-existing Ad5 immunity than a comparable Ad5 vector. These findings indicate that an Ad5/35 vector-based HIV vaccine may be of considerable value for clinical use. |
| Databáze: | OpenAIRE |
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