The Inner Annulus Fibrosus Encroaches on the Nucleus Pulposus in the Injured Mouse Tail Intervertebral Disc
| Autor: | Lutian Yao, Snehal S. Shetye, Yulong Wei, Yejia Zhang, Robert J. Tower, Robert L. Mauck, Sarah E. Gullbrand, Zuozhen Tian, Ling Qin |
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| Rok vydání: | 2020 |
| Předmět: |
Tail
musculoskeletal diseases 030506 rehabilitation Nucleus Pulposus Adhesion (medicine) Physical Therapy Sports Therapy and Rehabilitation Intervertebral Disc Degeneration Cell morphology Article Mice 03 medical and health sciences 0302 clinical medicine medicine Animals Annulus (mycology) business.industry Rehabilitation Annulus Fibrosus Intervertebral disc Anatomy musculoskeletal system medicine.disease Disease Models Animal medicine.anatomical_structure Fibrocartilage Cellular Morphology 0305 other medical science business Nucleus 030217 neurology & neurosurgery Immunostaining |
| Zdroj: | Am J Phys Med Rehabil |
| ISSN: | 1537-7385 0894-9115 |
| Popis: | OBJECTIVE The aim was to identify the source of cells within the center of the abnormal fibrocartilage tissue of the degenerative intervertebral disc after injury. DESIGN Cross-breeding of mice with an inducible type II promoter collagen construct (Col2CreER) to Rosa26-TdTomato mice has been shown to result in Cre-recombinase activity and Tomato expression in inner annulus fibrosus cells after tamoxifen injection. To investigate the role of the inner annulus fibrosus in the intervertebral disc injury response, tail intervertebral discs of Col2CreER/tdTomato mice were punctured with a needle and examined 1-4 wks after injury. N-cadherin was examined by immunostaining. RESULTS After the injury, the fibrocartilage in the degenerative intervertebral disc consisted of residual diseased nucleus pulposus cells and encroaching inner annulus fibrosus cells. The residual nucleus pulposus cells had lost their epithelial cell-like morphology and instead became oval shaped, with reduced adhesion to neighboring nucleus pulposus cells. This change in cellular morphology coincided with a loss of N-cadherin, which contributes to maintenance of healthy nucleus pulposus cell morphology. As expected, injured tail intervertebral discs showed reduced compressive properties as determined by biomechanical assessments. CONCLUSIONS The cellular composition of the degenerative intervertebral disc has been defined here, which is an important step in developing future treatments. |
| Databáze: | OpenAIRE |
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