Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk
| Autor: | Bianca Castiglioni, Stefano Morandi, Ana Carolina Alves, Emanuele Capra, Geraldo Márcio da Costa, Márcio Garcia Ribeiro, Milena Brasca, Milena Povolo, Paola Cremonesi, Mario Luini, Tiziana Silvetti |
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| Přispěvatelé: | Italian National Research Council (CNR ISPA), Italian National Research Council (CNR IBBA), Council for Agricultural Research and Economics – Research Centre for Animal Production and Aquaculture (CREA-ZA), Universidade Estadual Paulista (Unesp), Federal University of Lavras, Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER) |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cow milk 030106 microbiology Prototheca Biology Subspecies Microbiology Prototheca blaschkeae 03 medical and health sciences DNA Algal Prototheca blaschkeae subsp. brasiliensis 28S ribosomal RNA RNA Ribosomal 28S Genotype Botany RNA Ribosomal 18S Animals Internal transcribed spacer Mastitis Bovine Phylogeny Ecology Evolution Behavior and Systematics Ssu rrna gene Protothecal bovine mastitis Base Composition Strain (chemistry) Fatty Acids Sequence Analysis DNA General Medicine protothecal bovine mastitis cow milk Milk 030104 developmental biology Cattle Female Brazil |
| Zdroj: | International journal of systematic and evolutionary microbiology (Online) 67 (2017): 3865–3871. doi:10.1099/ijsem.0.002209 info:cnr-pdr/source/autori:Morandi Stefano; Cremonesi Paola; Povolo Milena; Capra Emanuele; Silvetti Tiziana; Castiglioni Bianca; Ribeiro Marcio Garcia; Alves Ana Carolina; da Costa Geraldo Marcio; Luini Mario; Brasca Milena/titolo:Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk./doi:10.1099%2Fijsem.0.002209/rivista:International journal of systematic and evolutionary microbiology (Online)/anno:2017/pagina_da:3865/pagina_a:3871/intervallo_pagine:3865–3871/volume:67 Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | Made available in DSpace on 2018-12-11T17:34:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-10-01 A strain of an achlorophyllic alga, named PR24T, was isolated from cow milk samples from the state of Minas Gerais, Brazil. Based on 18S rDNA, 28S rRNA, D1/D2 region of the LSU rDNA and SSU rRNA gene sequence similarities, this strain was found to be a member of the genus Prototheca and closely related to Prototheca blaschkeae SAG2064T. However, the novel strain could easily be distinguished from recognized Prototheca species by internal transcribed spacer, species-specific PCR, single-strand conformation polymorphism-PCR analysis and phenotypic characteristics. The inability to grow in Sabouraud broth at pH 4.0 and the different cellular fatty acid composition clearly distinguished PR24T from the reference strain of P. blaschkeae. The combination of genotypic and phenotypic data indicates that strain PR24T represents a subspecies of P. blaschkeae, for which the name Prototheca blaschkeae subsp. brasiliensis subsp. nov. is proposed. The respective type strain is PR24T (=DSM 103592T=IHEM 26958T). Institute of Sciences of Food Production Italian National Research Council (CNR ISPA), Via Celoria 2 Institute of Agricultural Biology and Biotechnology Italian National Research Council (CNR IBBA), Via Einstein Council for Agricultural Research and Economics – Research Centre for Animal Production and Aquaculture (CREA-ZA), Via Lombardo 11 Department of Veterinary Hygiene and Public Health Universidade Estadual Paulista (UNESP) Department of Veterinary Medicine Federal University of Lavras Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), Via Einstein Department of Veterinary Hygiene and Public Health Universidade Estadual Paulista (UNESP) |
| Databáze: | OpenAIRE |
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