Glycodendritic structures based on Boltorn hyperbranched polymers and their interactions with Lens culinaris lectin
| Autor: | Pedro M. Nieto, Díaz, Antonio Bernad, Arce E, Castro Rg, Javier Rojo |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Magnetic Resonance Spectroscopy
Cell Survival Polymers Hyperbranched polymers Biomedical Engineering Pharmaceutical Science Mannose Bioengineering Ligands Cell Line Mannans chemistry.chemical_compound Chlorocebus aethiops Animals Humans Solubility Pharmacology Biological studies Low toxicity biology Dose-Response Relationship Drug Molecular Structure Chemistry Mannose binding Organic Chemistry Lectin Stereoisomerism Biochemistry Models Chemical COS Cells Biophysics biology.protein Lens culinaris lectin Plant Lectins Biotechnology |
| Zdroj: | Bioconjugate chemistry. 14(4) |
| ISSN: | 1043-1802 |
| Popis: | Multivalent scaffolds bearing carbohydrates have been prepared to mediate biological processes where carbohydrates are involved. These systems consist of dendritic structures based on Boltorn H20 and H30 hyperbranched polymers to which carbohydrates are linked through a convenient spacer. Mannose has been chosen as a sugar unit to test the viability of this strategy. These glycodendritic compounds have been prepared in a few steps with good yields, showing a high solubility in physiological media and low toxicity. The binding of these dendritic polymers to the mannose-binding lectin Lens culinaris (LCA) was studied using STD-NMR experiments and quantitative precipitation assays. The results demonstrate the existence of a clear interaction between the mannose derivative systems and the Lens lectin where the dendritic scaffold does not have an important role in mannose binding but supplies the necessary multivalence for lectin cluster formation. These glycodendritic structures are able to interact with a receptor, and therefore they can be considered as promising tools for biological studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|