Reactive oxygen species generation in peripheral blood monocytes and oxidized LDL are increased in hyperlipidemic patients
| Autor: | Lucia Nassi Castilho, Eliana Cotta de Faria, Giovanna R. Degasperi, Edilma Maria de Albuquerque Vasconcelos, Anibal E. Vercesi, Helena C. F. Oliveira |
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| Rok vydání: | 2009 |
| Předmět: |
Adult
Male medicine.medical_specialty Lipoproteins medicine.medical_treatment Clinical Biochemistry Hyperlipidemias medicine.disease_cause Monocytes Flow cytometry Internal medicine Hyperlipidemia medicine Humans chemistry.chemical_classification Reactive oxygen species medicine.diagnostic_test Chemistry Insulin Monocyte General Medicine Middle Aged medicine.disease In vitro Lipoproteins LDL Endocrinology medicine.anatomical_structure Immunology Female Reactive Oxygen Species Lipid profile Oxidative stress |
| Zdroj: | Clinical Biochemistry. 42:1222-1227 |
| ISSN: | 0009-9120 |
| DOI: | 10.1016/j.clinbiochem.2009.05.010 |
| Popis: | Objectives Experimental and in vitro evidences have established that reactive oxygen species (ROS) generated by vascular wall cells play a key role in atherogenesis. Here, we evaluated the rate of ROS generation by resting peripheral monocytes in naive hyperlipidemic subjects. Design and methods Primary hypercholesterolemic, combined hyperlipidemic, and normolipidemic individuals were studied. ROS generation and the mitochondrial electrical transmembrane potential were estimated by flow cytometry. Plasma oxidized (ox) LDL levels and lipid profile were measured by ELISA and enzymatic colorimetric methods. Results Both hyperlipidemic groups presented significantly higher rates of monocyte ROS generation and elevated plasma levels of ox-LDL. Combined hyperlipidemic subjects presented increased levels of small dense LDL and insulin. Significant positive correlations between monocyte ROS generation and ox-LDL concentrations were found in pooled data. Conclusions These data provide evidence that ROS production by circulating monocytes from hyperlipidemic subjects may contribute to the systemic oxidative stress and possibly to atherogenesis. |
| Databáze: | OpenAIRE |
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