Extraction of bioactive compounds against cardiovascular diseases from Lentinula edodes using a sequential extraction method
| Autor: | Cristina Soler-Rivas, Diego Morales, Adriana Jimenez Piris, M. Prodanov, Alejandro Ruiz-Rodríguez |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Comunidad de Madrid, Morales, Diego [0000-0002-4600-6673], Morales, Diego |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
DPPH Shiitake Mushrooms Angiotensin-Converting Enzyme Inhibitors Chemical Fractionation Antioxidants 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology Picrates Antioxidant activity Humans Benzothiazoles Eritadenine 030109 nutrition & dietetics Chromatography ABTS Supercritical CO2 extraction biology Extraction (chemistry) Biphenyl Compounds Supercritical fluid extraction 04 agricultural and veterinary sciences biology.organism_classification 040401 food science Hot water extraction Lentinula Cholesterol chemistry Cardiovascular Diseases Shiitake mushrooms Sulfonic Acids Lenthionine Biotechnology |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | Three extraction methods were sequentially combined to obtain fractions from Lentinula edodes (shiitake mushrooms) containing bioactive compounds against cardiovascular diseases (CVDs). Fruiting bodies were first extracted with plain water, obtained residue was then submitted to supercritical fluid extraction (SFE) and remaining residue submitted to hot water extraction. Sequential design allowed reutilization of the nonextracted material as raw material for the successive extractions increasing extraction yields and separating interesting compounds. Obtained fractions contained different amounts of ß-glucans, chitins, eritadenine, lenthionine, ergosterol, proteins/peptides and phenolic compounds conferring them different bioactivities. Water soluble fractions showed high antioxidant activities (ABTS+• and DPPH• scavenging capacity and reducing power), they were also able to inhibit one of the main enzymes involved in hypertension (angiotensin-I converting enzyme) and the key enzyme of cholesterol metabolism (3-hydroxy-3-methylglutaryl coenzyme A reductase). The latter inhibitory activity was also noticed in SFE extracts although ergosterol and other lipid-like molecules were isolated. Dietary fibers were separated in the third extraction. Therefore, with this sequential extraction procedure bioactive compounds against CVDs can be selectively separated from a single batch of shiitake powder. The research was supported by AGL2014–56211 national R 1 D program from the Spanish Ministry of Science and Innovation and S2013/ABI-2728 regional program from the Community of Madrid (Spain). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|