Proteomic and transcriptomic studies of BGC823 cells stimulated with Helicobacter pylori isolates from gastric MALT lymphoma

Autor: Lihua He, Jianzhong Zhang, Fanliang Meng, Huifang Zhang, Di Xiao, Qing-Hua Zou
Rok vydání: 2020
Předmět:
0301 basic medicine
Molecular transducer activity
Proteome
Gene Expression
Pathology and Laboratory Medicine
Biochemistry
Transcriptome
0302 clinical medicine
Cell Signaling
Helicobacter
Medicine and Health Sciences
Wnt Signaling Pathway
WNT Signaling Cascade
Multidisciplinary
biology
Incidence
Wnt signaling pathway
Genomics
Signaling Cascades
Bacterial Pathogens
Oncology
Signal transducer activity
Medical Microbiology
030220 oncology & carcinogenesis
Medicine
Pathogens
Transcriptome Analysis
Research Article
Signal Transduction
China
Science
Microbiology
Helicobacter Infections
03 medical and health sciences
Gene Types
Diagnostic Medicine
Stomach Neoplasms
Gastrointestinal Tumors
Genetics
Cancer Detection and Diagnosis
Humans
Microbial Pathogens
Hippo signaling pathway
Bacteria
Helicobacter pylori
Gene Expression Profiling
Organisms
Biology and Life Sciences
Cancers and Neoplasms
Computational Biology
Cell Biology
Lymphoma
B-Cell
Marginal Zone
Genome Analysis
biology.organism_classification
Gene expression profiling
Gastric Cancer
030104 developmental biology
Cancer research
Regulator Genes
Biomarkers
Molecular function regulator
Zdroj: PLoS ONE, Vol 15, Iss 9, p e0238379 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: BackgroundThe correlation between the infection of H. pylori and the occurrence of gastric MALT lymphoma (GML) has been well documented. However, the mechanism of how GML is caused by this bacterium is not well understood, although some immunologic mechanisms are thought to be involved.Materials and methodsIn this study, we performed both transcriptomic and proteomic analyses on gastric cancer cells infected by H. pylori isolates from GML patients and the gastric ulcer strain 26695 to investigate the differentially expressed molecular signatures that were induced by GML isolates.ResultsTranscriptomic analyses revealed that the differentially expressed genes (DEGs) were mainly related to binding, catalytic activity, signal transducer activity, molecular transducer activity, nucleic acid binding transcription factor activity, and molecular function regulator. Fifteen pathways, including the Wnt signaling pathway, the mTOR signaling pathway, the NOD-like receptor signaling pathway and the Hippo signaling pathway, were revealed to be related to GML isolates. Proteomic analyses results showed that there were 116 differentially expressed proteins (DEPs). Most of these DEPs were associated with cancer, and 29 have been used as biomarkers for cancer diagnosis. We also found 63 upstream regulators that can inhibit or activate the expression of the DEPs. Combining the proteomic and transcriptomic analyses revealed 12 common pathways. This study provides novel insights into H. pylori-associated GML. The DEPs we found may be good candidates for GML diagnosis and treatment.ConclusionsThis study revealed specific pathways related to GML and potential biomarkers for GML diagnosis.
Databáze: OpenAIRE
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