Biochemical Characteristics of Rat C-Type Virus WF-1
| Autor: | Jack Bookout, Harold G. Haines, David L. Bronson, Victor V. Bergs |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Hot Temperature
Sucrose biology DNA polymerase viruses Buoyant density RNA RNA-Directed DNA Polymerase Molecular biology General Biochemistry Genetics and Molecular Biology Virus Reverse transcriptase Rats Molecular Weight Sedimentation coefficient chemistry.chemical_compound Retroviridae chemistry Centrifugation Density Gradient biology.protein Animals RNA Viral Dimethyl Sulfoxide Viral rna |
| Zdroj: | Experimental Biology and Medicine. 152:116-121 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | SummaryThe non-oncogenic rat C-type virus WF-1, isolated from a Wistar-Furth rat embryo cell line, was characterized biochemically. The purified virus has a buoyant density of 1.15 to 1.16 g/cm3 in sucrose, RNA-dependent DNA polymerase (reverse transcriptase) activity, and RNA with a sedimentation coefficient of 62 to 68 S. The viral RNA is single-stranded, and, upon treatment with heat, yields components with sedimentation coefficients of 36 S, 18 to 20 S, and 4 to 12 S. |
| Databáze: | OpenAIRE |
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