Cytoplasmic dynein is required for normal nuclear segregation in yeast
| Autor: | J C Jauniaux, J C van Vliet-Reedijk, L A Urrestarazu, R J Planta, D Eshel, S Vissers, I R Gibbons |
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| Rok vydání: | 1993 |
| Předmět: |
Cytoplasm
Multidisciplinary Cell division biology Sequence Homology Amino Acid Saccharomyces cerevisiae Dynein Genes Fungal Molecular Sequence Data Restriction Mapping Chromosome Mapping Dyneins Mitosis biology.organism_classification Spindle apparatus Cell biology Mutagenesis Insertional Dynein ATPase Microtubule Amino Acid Sequence Sequence Alignment Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 90(23) |
| ISSN: | 0027-8424 |
| Popis: | We have identified the gene DYN1, which encodes the heavy chain of cytoplasmic dynein in the yeast Saccharomyces cerevisiae. The predicted amino acid sequence (M(r) 471,305) reveals the presence of four P-loop motifs, as in all dyneins known so far, and has 28% overall identity to the dynein heavy chain of Dictyostelium [Koonce, M. P., Grissom, P. M. & McIntosh, J. R. (1992) J. Cell Biol. 119, 1597-1604] with 40% identity in the putative motor domain. Disruption of DYN1 causes misalignment of the spindle relative to the bud neck during cell division and results in abnormal distribution of the dividing nuclei between the mother cell and the bud. Cytoplasmic dynein, by generating force along cytoplasmic microtubules, may play an important role in the proper alignment of the mitotic spindle in yeast. |
| Databáze: | OpenAIRE |
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