Analysis of the Major Probiotics in Healthy Women’s Breast Milk by Realtime PCR. Factors Affecting the Presence of Those Bacteria
| Autor: | Panagiotis Halvatsiotis, Georgia Nikolopoulou, Jan Van Impe, Nikolaos Genaris, Anthimia Batrinou, Efstathia Tsakali, Theofania Tsironi, Ekaterini Petropoulou, Dimitra Houhoula, Apollon Thomas, Despina Vougiouklaki, Dionyssios Antonopoulos, Anastasia Tsilia |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Technology
Chemistry Multidisciplinary Physiology Gut flora BIFIDOBACTERIA law.invention Probiotic Engineering fluids and secretions law Lactation Lactobacillus General Materials Science Biology (General) Instrumentation Bifidobacterium 2. Zero hunger Fluid Flow and Transfer Processes probiotic bacteria 0303 health sciences biology Physics Incidence (epidemiology) General Engineering food and beverages Engineering (General). Civil engineering (General) MICROBIOTA 3. Good health Computer Science Applications Chemistry PREGNANCY medicine.anatomical_structure Physical Sciences breast milk TA1-2040 QH301-705.5 QC1-999 Materials Science Engineering Multidisciplinary Materials Science Multidisciplinary Breast milk LACTIC-ACID BACTERIA Physics Applied 03 medical and health sciences medicine QD1-999 030304 developmental biology Science & Technology realtime PCR IDENTIFICATION Lactobacillus spp 030306 microbiology Process Chemistry and Technology biology.organism_classification Bifidobacterium spp Colostrum |
| Zdroj: | Applied Sciences, Vol 11, Iss 9400, p 9400 (2021) Applied Sciences Volume 11 Issue 20 |
| ISSN: | 2076-3417 |
| DOI: | 10.3390/app11209400 |
| Popis: | Breast milk has been reported as a bacteria source that affects infant gut microbiota development. The present study utilizes a realtime PCR method to identify Lactobacillus and Bifidobacterium spp. in the breast milk of healthy women and attempts to identify factors affecting those human milk bacteria. Breast milk samples—both colostrum and mature milk—of 100 healthy women, were collected in Greece along with data about the demographic factors and nutritional habits of the volunteers. The colostrum samples were found to have higher percentages of either Bifidobacterium or Lactobacillus (76.9% and 48.6%, respectively) compared to the mature milk samples. For younger women, aged from 18 to 29 years, and women from rural areas, bacteria were detected in higher incidence than for older groups and women in urban areas, respectively. Moreover, for high-BMI women, bacteria were detected in lower incidence than for those with normal BMI. Probiotic supplements did not affect the composition of the breast milk-identified bacteria. Various factors such as lactation stage, maternal age, maternal weight, and residential location may contribute to the presence of those species in human milk. RT PCR has significant potential for the microbiological analysis of human milk. |
| Databáze: | OpenAIRE |
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