Differential expression of Tie2 receptor and VEGFR2 by endothelial clones derived from isolated bovine mononuclear cells
| Autor: | Brenda L. Coomber, Alexander Yurkiewich, Una Adamcic |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
CD31
Pathology Anatomy and Physiology Heredity Angiogenesis Receptor expression lcsh:Medicine Gene Expression 030204 cardiovascular system & hematology Cardiovascular Cardiovascular System Mice 0302 clinical medicine Cell Movement Molecular Cell Biology lcsh:Science Cells Cultured 0303 health sciences Multidisciplinary Neovascularization Pathologic Stem Cells Receptor TIE-2 Endothelial stem cell Adult Stem Cells Phenotypes embryonic structures cardiovascular system Medicine Stem cell Cellular Types Research Article medicine.medical_specialty Biology Mural cell Molecular Genetics 03 medical and health sciences Vascular Biology Diagnostic Medicine medicine Genetics Animals Progenitor cell 030304 developmental biology Matrigel lcsh:R Endothelial Cells Computational Biology Molecular biology Vascular Endothelial Growth Factor Receptor-2 Leukocytes Mononuclear Leukocyte Common Antigens lcsh:Q Cattle Biomarkers Developmental Biology General Pathology |
| Zdroj: | PLoS ONE PLoS ONE, Vol 7, Iss 12, p e53385 (2012) |
| ISSN: | 1932-6203 |
| Popis: | The purpose of these experiments was to evaluate the expression of endothelial markers, such as Tie2 and VEGFR2 in endothelial cells derived from blood mononuclear endothelial progenitor cells. Bovine mononuclear cells were isolated using separation by centrifugation and were grown in endothelial specific media supplemented with growth factors. Isolation of the whole cell population of mononuclear cells (MNC) from bovine peripheral blood gave rise to progenitor-like cells (CD45(-)) that, although morphologically similar, have different phenotypes revealed by expression of endothelial specific markers Tie2 and VEGFR2. Plating of MNCs on collagen and fibronectin gave rise to more colonies than non-coated dishes. Occasional colonies from MNC isolations had a mural cell phenotype, negative for Tie2 and VEGFR2 but positive for smooth muscle actin and PDGFRβ. Although cells expressing high levels of VEGFR2 and low levels of Tie2, and vice versa were both able to form cords on Matrigel, cells with higher expression of Tie2 migrate faster in a scratch assay than ones with lower expression of Tie2. When these different clones of cells were introduced in mice through tail vein injections, they retained an ability to home to angiogenesis occurring in a subcutaneous Matrigel plug, regardless of their Tie2/VEGFR2 receptor expression patterns, but cells with high VEGFR2/low Tie2 were more likely to be CD31 positive. Therefore, we suggest that active sites of angiogenesis (such as wounds, tumors, etc.) can attract a variety of endothelial cell precursors that may differentially express Tie2 and VEGFR2 receptors, and thus affect our interpretation of EPCs as biomarkers or therapies for vascular disease. |
| Databáze: | OpenAIRE |
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