Comparison of adhesin genes expression among Klebsiella oxytoca ESBL-non-producers in planktonic and biofilm mode of growth, and imipenem sublethal exposure
| Autor: | Shahrazad M.J. Al-Shadeedi, Suhad Saad Mahmood, Abdolmajid Ghasemian, Miaad K. Alkhudhairy, Sana A. Albustan |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Imipenem medicine.drug_class 030106 microbiology Antibiotics Fimbria Microbial Sensitivity Tests Biology beta-Lactams Microbiology beta-Lactamases 03 medical and health sciences Gene expression medicine Humans Adhesins Bacterial Gene Klebsiella oxytoca Biofilm Gene Expression Regulation Bacterial biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Anti-Bacterial Agents Klebsiella Infections Bacterial adhesin Phenotype 030104 developmental biology Infectious Diseases Carbapenems Genes Bacterial Biofilms bacteria medicine.drug |
| Zdroj: | Microbial Pathogenesis. 134:103558 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2019.103558 |
| Popis: | Background Bacterial adhesins play an important role in the bacterial attachment and colonization. The aim of this study was comparison of adhesin genes expression in the planktonic and biofilm mode of growth among ESBL-non-producers isolates of K. oxytoca and effect of imipenem. Materials and methods A total of eight extended-spectrum beta-lactamase (ESBL) non-producer K. oxytoca isolates were included from patients with hemorrhagic colitis. The adhesin genes including fimA (type 1 fimbria), mrkA (type 3 fimbria), pilQ and the capsular matB genes were adopted. Phenotypic biofilm production was assessed by microtiter tissue plate assay. Expression of adhesin genes in the planktonic and biofilm growth conditions was calculated using quantitative Real-time PCR (RT-qPCR) technique and sub-MIC (0.25 μg/ml) levels of imipenem were also added to broth culture of isolates to evaluate the gene expression. Results The isolates produced biofilm in moderate level. The expression of pilQ, mrkA and matB but not fimA genes was significantly higher in biofilm conditions compared to the planktonic mode of growth (p = 0.002, p = 0.011 and p = 001, respectively). In addition, imipenem sub-MIC treatment led to a significant overexpression of matB (p = 0.002) and mrkA (p = 0.003) genes compared to the control group. Conclusion Although none of isolates produced strong biofilm, biofilm conditions led to the increase in the expression of adhesin encoding genes in non-ESBL-producing K. oxytoca. Furthermore, β-lactams; and especially carbapenems possibly increase the colonization of K. oxytoca and increase the biofilm formation. Hence, accurate consumption of antibiotics must be considered. |
| Databáze: | OpenAIRE |
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