Herpes simplex virus type 1 penetrates the basement membrane in human nasal respiratory mucosa

Autor: Wim Van den Broeck, Sarah Glorieux, Siska Croubels, Lennert Steukers, Hans Nauwynck, Annelies P. Vandekerckhove, Anamaria Rekecki, Reginald Clayton, Herman W. Favoreel, Joline Goossens, Claus Bachert
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Respiratory Mucosa
Pathology
Viral Diseases
Pulmonology
GENITAL-INFECTION
Mucous membrane of nose
Herpesvirus 1
Human
medicine.disease_cause
DISEASE
Basement Membrane
Epithelium
VIVO
Molecular Cell Biology
Multidisciplinary
US3 PROTEIN-KINASE
FLOW-CYTOMETRY
Extracellular Matrix
Host-Pathogen Interaction
medicine.anatomical_structure
Infectious Diseases
ORGAN-CULTURE
Medicine
EXPLANTS
Research Article
medicine.medical_specialty
Stromal cell
BLOCKS APOPTOSIS
Viral Entry
Clinical Research Design
Science
Biology
Microbiology
Models
Biological
Fluorescence
Stromal Invasion
Virology
medicine
Upper Respiratory Tract Infections
In Situ Nick-End Labeling
Humans
Veterinary Sciences
Basement membrane
Lamina propria
Modeling
Herpes Simplex
IN-VITRO
Nasal Mucosa
Herpes simplex virus
Otorhinolaryngology
CELLS
Respiratory Infections
Nasal Diseases
Infectious Disease Modeling
Viral Transmission and Infection
Zdroj: PLoS ONE, Vol 6, Iss 7, p e22160 (2011)
PLoS ONE
PLOS ONE
ISSN: 1932-6203
Popis: BackgroundHerpes simplex virus infections are highly prevalent in humans. However, the current therapeutics suffer important drawbacks such as limited results in neonates, increasing occurrence of resistance and impeded treatment of stromal infections. Remarkably, interactions of herpesviruses with human mucosa, the locus of infection, remain poorly understood and the underlying mechanisms in stromal infection remain controversial.Methodology/principal findingsA human model consisting of nasal respiratory mucosa explants was characterised. Viability and integrity were examined during 96 h of cultivation. HSV1-mucosa interactions were analysed. In particular, we investigated whether HSV1 is able to reach the stroma. Explant viability and integrity remained preserved. HSV1 induced rounding up and loosening of epithelial cells with very few apoptotic and necrotic cells observed. Following 16-24 h of infection, HSV1 penetrated the basement membrane and replicated in the underlying lamina propria.Conclusions/significanceThis human explant model can be used to study virus-mucosa interactions and viral mucosal invasion mechanisms. Using this model, our results provide a novel insight into the HSV1 stromal invasion mechanism and for the first time directly demonstrate that HSV1 can penetrate the basement membrane.
Databáze: OpenAIRE
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