FOXM 1 induces Vasculogenic mimicry in esophageal cancer through β-catenin /Tcf4 signaling
| Autor: | Xiaoying Tao, Lili Cheng, Qi Wang, Dafang Zheng, Qiong Wu, Dongbing Lu, Zhiwei Wang, Jia Ma, Damin Chai, Zenong Cheng, Shiwu Wu, Hongfei Ci, Danna Wang, Yong Zhang, Yanzi Qin, Yisheng Tao |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Histology Esophageal Neoplasms β-Catenin VM Esophageal cancer Proliferation Biology medicine.disease_cause Pathology and Forensic Medicine 03 medical and health sciences Transcription Factor 4 0302 clinical medicine Invasion Cell Movement Cell Line Tumor lcsh:Pathology medicine Humans Gene silencing Neoplasm Invasiveness Vasculogenic mimicry Migration beta Catenin Tcf4 Aged Cell Proliferation Cell growth Research Forkhead Box Protein M1 FOXM1 Cell migration General Medicine Middle Aged Cadherins Gene Expression Regulation Neoplastic 030104 developmental biology Cell culture 030220 oncology & carcinogenesis Catenin Cancer research Female Carcinogenesis lcsh:RB1-214 |
| Zdroj: | Diagnostic Pathology Diagnostic Pathology, Vol 15, Iss 1, Pp 1-14 (2020) |
| ISSN: | 1746-1596 |
| DOI: | 10.1186/s13000-020-00929-9 |
| Popis: | Objective To investigate the role of FOXM1, β-catenin and TCF4 in esophageal cancer (EC) and their relationship to VM (Vasculogenic Mimicry). Methods CCK-8 were performed to examine EC cell proliferation in FOXM1 silenced cells. EC cell migration and invasion were investigated through wound healing and Transwell assays, respectively. The formation of pipe like structures were assessed in 3D cultures. The expression of Foxm1, β-catenin, Tcf4 and E-cadherin were investigated through western blot, RT-qPCR and immunohistochemistry (IHC) staining. The relationship between FOXM1 expression, clinic-pathological features, and overall survival (OS) were further analyzed. Results A loss of FOXM1 expression correlated with the OS of ESCC patients. FOXM1 silencing led to a loss of cell growth and suppressed cell migration and invasion in ESCC cells. VM structures were identified in ESCC tissues and human EC cell lines. Mechanistically, FOXM1 was found to promote tumorigenesis through the regulation of β-catenin, Tcf4, and E-cadherin in EC cells, leading to the formation of VM structures. Conclusions These findings highlight FoxM1 as a novel therapeutic target in ESCC. |
| Databáze: | OpenAIRE |
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