The Antiviral Effect of the Chemical Compounds Targeting DED/EDh Motifs of the Viral Proteins on Lymphocytic Choriomeningitis Virus and SARS-CoV-2
| Autor: | Shuzo Urata, Takeshi Ishikawa, Kouichi Morita, Mya Myat Ngwe Tun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
viruses 030106 microbiology Amino Acid Motifs Biology Viral Nonstructural Proteins Lymphocytic choriomeningitis medicine.disease_cause Virus Replication Antiviral Agents Microbiology Virus Article 03 medical and health sciences Exon Viral Proteins antivirals Virology Chlorocebus aethiops Drug Discovery medicine Animals Humans Lymphocytic choriomeningitis virus Vero Cells Virus classification DED/EDh motif Lymphocytic choriomeningitis virus (LCMV) SARS-CoV-2 virus diseases medicine.disease QR1-502 exonuclease (ExoN) motif Molecular Docking Simulation 030104 developmental biology Infectious Diseases Lassa virus Viral replication Cell culture A549 Cells Exoribonucleases Vero cell |
| Zdroj: | Viruses, Vol 13, Iss 1220, p 1220 (2021) Viruses Volume 13 Issue 7 |
| ISSN: | 1999-4915 |
| Popis: | Arenaviruses and coronaviruses include several human pathogenic viruses, such as Lassa virus, Lymphocytic choriomeningitis virus (LCMV), SARS-CoV, MERS-CoV, and SARS-CoV-2. Although these viruses belong to different virus families, they possess a common motif, the DED/EDh motif, known as an exonuclease (ExoN) motif. In this study, proof-of-concept studies, in which the DED/EDh motif in these viral proteins, NP for arenaviruses, and nsp14 for coronaviruses, could be a drug target, were performed. Docking simulation studies between two structurally different chemical compounds, ATA and PV6R, and the DED/EDh motifs in these viral proteins indicated that these compounds target DED/EDh motifs. The concentration which exhibited modest cell toxicity was used with these compounds to treat LCMV and SARS-CoV-2 infections in two different cell lines, A549 and Vero 76 cells. Both ATA and PV6R inhibited the post-entry step of LCMV and SARS-CoV-2 infection. These studies strongly suggest that DED/EDh motifs in these viral proteins could be a drug target to combat two distinct viral families, arenaviruses and coronaviruses. Viruses, 13(7), art. no. 1220; 2021 |
| Databáze: | OpenAIRE |
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