Goreisan Prevents Brain Edema after Cerebral Ischemic Stroke by Inhibiting Aquaporin 4 Upregulation in Mice
| Autor: | Takashi Egawa, Yoshihiko Nakamura, Hiroyasu Ishikura, Chisa Nishigami, Hidetoshi Kamimura, Koji Tominaga, Kazunori Sano, Keiichi Irie, Yuta Yamashita, Kenichi Mishima, Koichi Matsuo, Takayuki Myose, Yutaka Shigemori, Takafumi Nakano |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Time Factors Kampo Central nervous system Ischemia Brain Edema Brain damage Motor Activity Pharmacology Mice 03 medical and health sciences 0302 clinical medicine Body Water Edema medicine Animals cardiovascular diseases Intracranial pressure Aquaporin 4 Behavior Animal business.industry Rehabilitation Brain Infarction Middle Cerebral Artery medicine.disease Up-Regulation Motor coordination Disease Models Animal Neuroprotective Agents 030104 developmental biology medicine.anatomical_structure Anesthesia Surgery Medicine Kampo sense organs Neurology (clinical) medicine.symptom Cardiology and Cardiovascular Medicine business 030217 neurology & neurosurgery Drugs Chinese Herbal |
| Zdroj: | Journal of Stroke and Cerebrovascular Diseases. 27:758-763 |
| ISSN: | 1052-3057 |
| DOI: | 10.1016/j.jstrokecerebrovasdis.2017.10.010 |
| Popis: | Background Aquaporin 4 (AQP4) is a water-selective transport protein expressed in astrocytes throughout the central nervous system. AQP4 level increases after cerebral ischemia and results in ischemic brain edema. Brain edema markedly influences mortality and motor function by elevating intracranial pressure that leads to secondary brain damage. Therefore, AQP4 is an important target to improve brain edema after cerebral ischemia. The Japanese herbal Kampo medicine, goreisan, is known to inhibit AQP4 activity. Here, we investigated whether goreisan prevents induction of brain edema by cerebral ischemia via AQP4 using 4-hour middle cerebral artery occlusion (4h MCAO) mice. Methods Goreisan was orally administered at a dose of 500 mg/kg twice a day for 5 days before MCAO. AQP4 expression and motor coordination were measured by Western blotting and rotarod test, respectively. Results Brain water content of 4h MCAO mice was significantly increased at 24 hours after MCAO. Treatment with goreisan significantly decreased both brain water content and AQP4 expression in the ischemic brain at 24 hours after MCAO. In addition, treatment with goreisan alleviated motor coordination deficits at 24 hours after MCAO. Conclusions The results of this study suggested that goreisan may be a useful new therapeutic option for ischemic brain edema. |
| Databáze: | OpenAIRE |
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