A Comprehensive Assessment of Apigenin as an Antiproliferative, Proapoptotic, Antiangiogenic and Immunomodulatory Phytocompound

Autor: Codruta Soica, Ioana Zinuca Pavel, Andrea Bor, Stefana Avram, Corina Danciu, Camelia Oprean, Claudia Farcas, István Zupkó, Alexandra Ghițu, Oana Duicu, Heinfried H. Radeke, Cristina Dehelean, Anja Schwiebs, Florina Bojin
Rok vydání: 2019
Předmět:
0301 basic medicine
Lipopolysaccharides
Anti-Inflammatory Agents
Angiogenesis Inhibitors
Apoptosis
Pharmacology
chemistry.chemical_compound
angiogenesis
0302 clinical medicine
Adenosine Triphosphate
Annexin
Cell Movement
Cytotoxic T cell
Apigenin
Melanoma
Nutrition and Dietetics
Chemistry
Caspase 3
Cell cycle
Mitochondria
030220 oncology & carcinogenesis
Cytokines
lcsh:Nutrition. Foods and food supply
proliferation
lcsh:TX341-641
Article
03 medical and health sciences
Magnoliopsida
Cell Line
Tumor
Humans
Immunologic Factors
Secretion
ddc:610
Cell Proliferation
Inflammation
L-Lactate Dehydrogenase
Cell growth
Plant Extracts
Cell Cycle Checkpoints
Dendritic Cells
Antineoplastic Agents
Phytogenic
Diet
A375 human melanoma cell line
030104 developmental biology
Cell culture
mitochondrial bioenergetics and glycolysis
Food Science
Phytotherapy
Zdroj: Nutrients
Nutrients, Vol 11, Iss 4, p 858 (2019)
Volume 11
Issue 4
ISSN: 2072-6643
Popis: Apigenin (4&prime
5,7-trihydroxyflavone) (Api) is an important component of the human diet, being distributed in a wide number of fruits, vegetables and herbs with the most important sources being represented by chamomile, celery, celeriac and parsley. This study was designed for a comprehensive evaluation of Api as an antiproliferative, proapoptotic, antiangiogenic and immunomodulatory phytocompound. In the set experimental conditions, Api presents antiproliferative activity against the A375 human melanoma cell line, a G2/M arrest of the cell cycle and cytotoxic events as revealed by the lactate dehydrogenase release. Caspase 3 activity was inversely proportional to the Api tested doses, namely 30 &mu
M and 60 &mu
M. Phenomena of early apoptosis, late apoptosis and necrosis following incubation with Api were detected by Annexin V-PI double staining. The flavone interfered with the mitochondrial respiration by modulating both glycolytic and mitochondrial pathways for ATP production. The metabolic activity of human dendritic cells (DCs) under LPS-activation was clearly attenuated by stimulation with high concentrations of Api. Il-6 and IL-10 secretion was almost completely blocked while TNF alpha secretion was reduced by about 60%. Api elicited antiangiogenic properties in a dose-dependent manner. Both concentrations of Api influenced tumour cell growth and migration, inducing a limited tumour area inside the application ring, associated with a low number of capillaries.
Databáze: OpenAIRE
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